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1imx

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(New page: 200px<br /> <applet load="1imx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1imx, resolution 1.82&Aring;" /> '''1.8 Angstrom crysta...)
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[[Image:1imx.gif|left|200px]]<br />
 
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<applet load="1imx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1imx, resolution 1.82&Aring;" />
 
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'''1.8 Angstrom crystal structure of IGF-1'''<br />
 
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==Overview==
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==1.8 Angstrom crystal structure of IGF-1==
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Despite efforts spanning considerably more than a decade, a, high-resolution view of the family of proteins known as insulin-like, growth factors (IGFs) has remained elusive. IGF-1 consists of three, helical segments which are connected by a 12-residue linker known as the, C-region. NMR studies of members of this family reveal a dynamic structure, with a topology resembling insulin but little structural definition in the, C-region. We have crystallized IGF-1 in the presence of the detergent, deoxy big CHAPS, and determined its structure at 1.8 A resolution by, multiwavelength anomalous diffraction, exploiting the anomalous scattering, of a single bromide ion and six of the seven sulfur atoms of IGF-1. The, structure reveals a well-defined conformation for much of the C-region, which extends away from the core of IGF-1 and has residues known to be, involved in receptor binding prominently displayed in a type II beta-turn., In the crystal, these residues form a dimer interface, but analytical, ultracentrifugation experiments demonstrate that at physiological, concentrations IGF-1 is monomeric. A single detergent molecule contacts, residues known to be important for IGF-1 binding protein (IGFBP), interactions. Biophysical and biochemical data show that the detergent, binds to IGF-1 specifically and blocks binding of IGFBP-1 and IGFBP-3.
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<StructureSection load='1imx' size='340' side='right'caption='[[1imx]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1imx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IMX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CPQ:N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE'>CPQ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1imx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imx OCA], [https://pdbe.org/1imx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1imx RCSB], [https://www.ebi.ac.uk/pdbsum/1imx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1imx ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/IGF1_HUMAN IGF1_HUMAN] Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:[https://omim.org/entry/608747 608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
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== Function ==
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[https://www.uniprot.org/uniprot/IGF1_HUMAN IGF1_HUMAN] The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.<ref>PMID:21076856</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/im/1imx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1imx ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known disease associated with this structure: Growth retardation with deafness and mental retardation due to IGF1 deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147440 147440]]
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*[[Insulin-like growth factor|Insulin-like growth factor]]
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== References ==
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==About this Structure==
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<references/>
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1IMX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with BR and CPQ as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IMX OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Crystal structure of human insulin-like growth factor-1: detergent binding inhibits binding protein interactions., Vajdos FF, Ultsch M, Schaffer ML, Deshayes KD, Liu J, Skelton NJ, de Vos AM, Biochemistry. 2001 Sep 18;40(37):11022-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11551198 11551198]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Deshayes, K.D.]]
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[[Category: Deshayes KD]]
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[[Category: Liu, J.]]
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[[Category: Liu J]]
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[[Category: Schaffer, M.L.]]
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[[Category: Schaffer ML]]
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[[Category: Skelton, N.J.]]
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[[Category: Skelton NJ]]
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[[Category: Ultsch, M.]]
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[[Category: Ultsch M]]
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[[Category: Vajdos, F.F.]]
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[[Category: Vajdos FF]]
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[[Category: Vos, A.M.de.]]
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[[Category: De Vos AM]]
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[[Category: BR]]
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[[Category: CPQ]]
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[[Category: detergent]]
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[[Category: insulin/relaxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:32:01 2007''
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Current revision

1.8 Angstrom crystal structure of IGF-1

PDB ID 1imx

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