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1itf

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[[Image:1itf.gif|left|200px]]<br /><applet load="1itf" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1itf" />
 
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'''INTERFERON ALPHA-2A, NMR, 24 STRUCTURES'''<br />
 
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==Overview==
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==INTERFERON ALPHA-2A, NMR, 24 STRUCTURES==
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The solution structure of recombinant human interferon alpha-2a (Roferon-A) has been determined by multidimensional heteronuclear NMR spectroscopy. The calculations using simulated annealing produced a family of 24 convergent structures which satisfy the experimental restraints comprising 1541 NOE-derived inter-proton distances, 187 dihedral restraints, 66 pairs of hydrogen bond restraints, and six upper and lower limits for two disulfide bridges. The fractional labeling of methyl groups allowed their direct and unambiguous stereospecific assignment which proved to be essential for obtaining a high resolution of the structures. A best fit superposition of residues 10 to 47, 50 to 101 and 111 to 157 gives an rms deviation of 0.62 A for the backbone heavy atoms and 1.39 A for all heavy atoms of these segments. The dominant feature of the structure is a cluster of five alpha-helices, four of which are arranged to form a left-handed helix bundle with an up-up-down-down topology and two over-hand connections. The interpretation of heteronuclear 15N- inverted question mark1H inverted question mark NOE data shows the co-existence of flexible regions within an otherwise rigid framework of the protein. Four stretches of pronounced flexibility can be located: Cys1-Ser8, Gly44-Ala50, Ile100-Lys112, and Ser160-Glu165. Among the structurally related four-helical bundle cytokines, the structure of IFN alpha-2a is most similar to that of human interferon alpha-2b and murine interferon-beta. From this structural information and mutagenesis data, areas on the surface of the protein are identified which seem to be important in receptor interactions.
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<StructureSection load='1itf' size='340' side='right'caption='[[1itf]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1itf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The August 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Interferons'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_8 10.2210/rcsb_pdb/mom_2010_8]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ITF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ITF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1itf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1itf OCA], [https://pdbe.org/1itf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1itf RCSB], [https://www.ebi.ac.uk/pdbsum/1itf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1itf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IFNA2_HUMAN IFNA2_HUMAN] Produced by macrophages, IFN-alpha have antiviral activities.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/1itf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1itf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ITF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ITF OCA].
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*[[Interferon 3D structures|Interferon 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The three-dimensional high resolution structure of human interferon alpha-2a determined by heteronuclear NMR spectroscopy in solution., Klaus W, Gsell B, Labhardt AM, Wipf B, Senn H, J Mol Biol. 1997 Dec 12;274(4):661-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9417943 9417943]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Interferons]]
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[[Category: Gsell, B.]]
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[[Category: Large Structures]]
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[[Category: Klaus, W.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Labhardt, A M.]]
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[[Category: Gsell B]]
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[[Category: Senn, H.]]
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[[Category: Klaus W]]
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[[Category: Wipf, B.]]
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[[Category: Labhardt AM]]
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[[Category: cytokine]]
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[[Category: Senn H]]
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[[Category: interferon]]
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[[Category: Wipf B]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:15:26 2008''
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INTERFERON ALPHA-2A, NMR, 24 STRUCTURES

PDB ID 1itf

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