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1j83

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[[Image:1j83.jpg|left|200px]]
 
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{{Structure
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==STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS==
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|PDB= 1j83 |SIZE=350|CAPTION= <scene name='initialview01'>1j83</scene>, resolution 1.70&Aring;
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<StructureSection load='1j83' size='340' side='right'caption='[[1j83]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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<table><tr><td colspan='2'>[[1j83]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_cellulovorans Clostridium cellulovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J83 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J83 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j83 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j83 OCA], [https://pdbe.org/1j83 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j83 RCSB], [https://www.ebi.ac.uk/pdbsum/1j83 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j83 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P94622_CLOCL P94622_CLOCL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j8/1j83_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j83 ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS'''
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==See Also==
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystal structure of the Clostridium cellulovorans carbohydrate-binding module (CBM) belonging to family 17 has been solved to 1.7 A resolution by multiple anomalous dispersion methods. CBM17 binds to non-crystalline cellulose and soluble beta-1,4-glucans, with a minimal binding requirement of cellotriose and optimal affinity for cellohexaose. The crystal structure of CBM17 complexed with cellotetraose solved at 2.0 A resolution revealed that binding occurs in a cleft on the surface of the molecule involving two tryptophan residues and several charged amino acids. Thermodynamic binding studies and alanine scanning mutagenesis in combination with the cellotetraose complex structure allowed the mapping of the CBM17 binding cleft. In contrast to the binding groove characteristic of family 4 CBMs, family 17 CBMs appear to have a very shallow binding cleft that may be more accessible to cellulose chains in non-crystalline cellulose than the deeper binding clefts of family 4 CBMs. The structural differences in these two modules may reflect non-overlapping binding niches on cellulose surfaces.
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==About this Structure==
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1J83 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_cellulovorans Clostridium cellulovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J83 OCA].
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==Reference==
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Recognition of cello-oligosaccharides by a family 17 carbohydrate-binding module: an X-ray crystallographic, thermodynamic and mutagenic study., Notenboom V, Boraston AB, Chiu P, Freelove AC, Kilburn DG, Rose DR, J Mol Biol. 2001 Dec 7;314(4):797-806. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11733998 11733998]
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[[Category: Cellulase]]
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[[Category: Clostridium cellulovorans]]
[[Category: Clostridium cellulovorans]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Boraston, A B.]]
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[[Category: Boraston AB]]
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[[Category: Chiu, P.]]
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[[Category: Chiu P]]
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[[Category: Freelove, A C.J.]]
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[[Category: Freelove ACJ]]
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[[Category: Kilburn, D G.]]
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[[Category: Kilburn DG]]
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[[Category: Notenboom, V.]]
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[[Category: Notenboom V]]
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[[Category: Rose, D R.]]
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[[Category: Rose DR]]
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[[Category: CA]]
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[[Category: carbohydrate binding module]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:59:54 2008''
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Current revision

STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS

PDB ID 1j83

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