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1j97

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[[Image:1j97.gif|left|200px]]
 
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{{Structure
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==Phospho-Aspartyl Intermediate Analogue of Phosphoserine phosphatase==
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|PDB= 1j97 |SIZE=350|CAPTION= <scene name='initialview01'>1j97</scene>, resolution 1.5&Aring;
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<StructureSection load='1j97' size='340' side='right'caption='[[1j97]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=BFD:ASPARTATE+BERYLLIUM+TRIFLUORIDE'>BFD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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<table><tr><td colspan='2'>[[1j97]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J97 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoserine_phosphatase Phosphoserine phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.3 3.1.3.3] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BFD:ASPARTATE+BERYLLIUM+TRIFLUORIDE'>BFD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0560 SerB]</span>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j97 OCA], [https://pdbe.org/1j97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j97 RCSB], [https://www.ebi.ac.uk/pdbsum/1j97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j97 ProSAT], [https://www.topsan.org/Proteins/BSGC/1j97 TOPSAN]</span></td></tr>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j97 OCA], [http://www.ebi.ac.uk/pdbsum/1j97 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1j97 RCSB]</span>
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</table>
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}}
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== Function ==
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[https://www.uniprot.org/uniprot/SERB_METJA SERB_METJA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j9/1j97_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j97 ConSurf].
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<div style="clear:both"></div>
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'''Phospho-Aspartyl Intermediate Analogue of Phosphoserine phosphatase'''
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==See Also==
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*[[Phosphoserine phosphatase|Phosphoserine phosphatase]]
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__TOC__
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==Overview==
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</StructureSection>
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Protein phosphoaspartate bonds play a variety of roles. In response regulator proteins of two-component signal transduction systems, phosphorylation of an aspartate residue is coupled to a change from an inactive to an active conformation. In phosphatases and mutases of the haloacid dehalogenase (HAD) superfamily, phosphoaspartate serves as an intermediate in phosphotransfer reactions, and in P-type ATPases, also members of the HAD family, it serves in the conversion of chemical energy to ion gradients. In each case, lability of the phosphoaspartate linkage has hampered a detailed study of the phosphorylated form. For response regulators, this difficulty was recently overcome with a phosphate analog, BeF(3)(-), which yields persistent complexes with the active site aspartate of their receiver domains. We now extend the application of this analog to a HAD superfamily member by solving at 1.5-A resolution the x-ray crystal structure of the complex of BeF(3)(-) with phosphoserine phosphatase (PSP) from Methanococcus jannaschii. The structure is comparable to that of a phosphoenzyme intermediate: BeF(3)(-) is bound to Asp-11 with the tetrahedral geometry of a phosphoryl group, is coordinated to Mg(2+), and is bound to residues surrounding the active site that are conserved in the HAD superfamily. Comparison of the active sites of BeF(3)(-) x PSP and BeF(3)(-) x CeY, a receiver domain/response regulator, reveals striking similarities that provide insights into the function not only of PSP but also of P-type ATPases. Our results indicate that use of BeF(3)(-) for structural studies of proteins that form phosphoaspartate linkages will extend well beyond response regulators.
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[[Category: Large Structures]]
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==About this Structure==
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1J97 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J97 OCA].
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==Reference==
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BeF(3)(-) acts as a phosphate analog in proteins phosphorylated on aspartate: structure of a BeF(3)(-) complex with phosphoserine phosphatase., Cho H, Wang W, Kim R, Yokota H, Damo S, Kim SH, Wemmer D, Kustu S, Yan D, Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8525-30. Epub 2001 Jul 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11438683 11438683]
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[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Phosphoserine phosphatase]]
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[[Category: Cho H]]
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[[Category: Single protein]]
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[[Category: Damo S]]
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[[Category: BSGC, Berkeley Structural Genomics Center.]]
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[[Category: Kim R]]
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[[Category: Cho, H.]]
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[[Category: Kim S-H]]
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[[Category: Damo, S.]]
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[[Category: Kustu S]]
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[[Category: Kim, R.]]
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[[Category: Wang W]]
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[[Category: Kim, S H.]]
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[[Category: Wemmer D]]
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[[Category: Kustu, S.]]
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[[Category: Yan D]]
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[[Category: Wang, W.]]
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[[Category: Yokota H]]
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[[Category: Wemmer, D.]]
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[[Category: Yan, D.]]
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[[Category: Yokota, H.]]
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[[Category: berkeley structural genomics center]]
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[[Category: beryllium fluoride]]
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[[Category: bsgc structure funded by nih]]
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[[Category: phospho-aspartyl]]
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[[Category: phosphoserine phosphatase]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: psp]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 05:53:54 2008''
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Current revision

Phospho-Aspartyl Intermediate Analogue of Phosphoserine phosphatase

PDB ID 1j97

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