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1j9o

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(New page: 200px<br /> <applet load="1j9o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j9o" /> '''SOLUTION STRUCTURE OF HUMAN LYMPHOTACTIN'''...)
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[[Image:1j9o.gif|left|200px]]<br />
 
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<applet load="1j9o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1j9o" />
 
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'''SOLUTION STRUCTURE OF HUMAN LYMPHOTACTIN'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF HUMAN LYMPHOTACTIN==
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Lymphotactin, the sole identified member of the C class of chemokines, specifically attracts T lymphocytes and natural killer cells. This, 93-residue protein lacks 2 of the 4 conserved cysteine residues, characteristic of the other 3 classes of chemokines and possesses an, extended carboxyl terminus, which is required for chemotactic activity. We, have determined the three-dimensional solution structure of recombinant, human lymphotactin by NMR spectroscopy. Under the conditions used for the, structure determination, lymphotactin was predominantly monomeric;, however, pulsed field gradient NMR self-diffusion measurements and, analytical ultracentrifugation revealed evidence of dimer formation., Sequence-specific chemical shift assignments were determined through, analysis of two- and three-dimensional NMR spectra of (15)N- and, (13)C/(15)N-enriched protein samples. Input for the torsion angle dynamics, calculations used in determining the structure included 1258 unique, NOE-derived distance constraints and 60 dihedral angle constraints, obtained from chemical-shift-based searching of a protein conformational, database. The ensemble of 20 structures chosen to represent the structure, had backbone and heavy atom rms deviations of 0.46 +/- 0.11 and 1.02 +/-, 0.14 A, respectively. The results revealed that human lymphotactin adopts, the conserved chemokine fold, which is characterized by a three-stranded, antiparallel beta-sheet and a C-terminal alpha-helix. Two regions are, dynamically disordered as evidenced by (1)H and (13)C chemical shifts and, [(15)N]-(1)H NOEs: residues 1-9 of the amino terminus and residues 69-93, of the C-terminal extension. A functional role for the C-terminal, extension, which is unique to lymphotactin, remains to be elucidated.
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<StructureSection load='1j9o' size='340' side='right'caption='[[1j9o]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1j9o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J9O FirstGlance]. <br>
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1J9O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1J9O OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j9o OCA], [https://pdbe.org/1j9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j9o RCSB], [https://www.ebi.ac.uk/pdbsum/1j9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j9o ProSAT]</span></td></tr>
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==Reference==
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</table>
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Monomeric solution structure of the prototypical 'C' chemokine lymphotactin., Kuloglu ES, McCaslin DR, Kitabwalla M, Pauza CD, Markley JL, Volkman BF, Biochemistry. 2001 Oct 23;40(42):12486-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11601972 11601972]
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== Function ==
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[https://www.uniprot.org/uniprot/XCL1_HUMAN XCL1_HUMAN] Chemotactic activity for lymphocytes but not for monocytes or neutrophils.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j9/1j9o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j9o ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kitabwalla, M.]]
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[[Category: Kitabwalla M]]
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[[Category: Kuloglu, E.S.]]
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[[Category: Kuloglu ES]]
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[[Category: Markley, J.L.]]
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[[Category: Markley JL]]
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[[Category: McCaslin, D.R.]]
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[[Category: McCaslin DR]]
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[[Category: Pauza, C.D.]]
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[[Category: Pauza CD]]
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[[Category: Volkman, B.F.]]
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[[Category: Volkman BF]]
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[[Category: chemokine]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:38:44 2007''
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Current revision

SOLUTION STRUCTURE OF HUMAN LYMPHOTACTIN

PDB ID 1j9o

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