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1jaj

From Proteopedia

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[[Image:1jaj.jpg|left|200px]]
 
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{{Structure
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==Solution Structure of DNA Polymerase X from the African Swine Fever Virus==
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|PDB= 1jaj |SIZE=350|CAPTION= <scene name='initialview01'>1jaj</scene>
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<StructureSection load='1jaj' size='340' side='right'caption='[[1jaj]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1jaj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/African_swine_fever_virus_BA71V African swine fever virus BA71V]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JAJ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE= O174L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10497 African swine fever virus])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jaj OCA], [https://pdbe.org/1jaj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jaj RCSB], [https://www.ebi.ac.uk/pdbsum/1jaj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jaj ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPOLX_ASFB7 DPOLX_ASFB7] Error-prone polymerase lacking a proofreading 3'-5' exonuclease which plays a role in viral DNA repair. Specifically binds intermediates in the single-nucleotide base-excision repair process. Also catalyzes DNA polymerization with low nucleotide-insertion fidelity. Together with the viral DNA ligase, fills the single nucleotide gaps generated by the AP endonuclease.<ref>PMID:12595253</ref> <ref>PMID:11685239</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ja/1jaj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jaj ConSurf].
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<div style="clear:both"></div>
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'''Solution Structure of DNA Polymerase X from the African Swine Fever Virus'''
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==See Also==
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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== References ==
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==Overview==
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<references/>
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DNA polymerase X (Pol X) from the African swine fever virus (ASFV) specifically binds intermediates in the single-nucleotide base-excision repair process, an activity indicative of repair function. In addition, Pol X catalyzes DNA polymerization with low nucleotide-insertion fidelity. The structural mechanisms by which DNA polymerases confer high or low fidelity in DNA polymerization remain to be elucidated. The three-dimensional structure of Pol X has been determined. Unlike other DNA polymerases, Pol X is formed from only a palm and a C-terminal subdomain. Pol X has a novel palm subdomain fold, containing a positively charged helix at the DNA binding surface. Purine deoxynucleoside triphosphate (dNTP) substrates bind between the palm and C-terminal subdomain, at a dNTP-binding helix, and induce a unique conformation in Pol X. The purine dNTP-bound conformation and high binding affinity for dGTP-Mg(2+) of Pol X may contribute to its low fidelity.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: African swine fever virus BA71V]]
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1JAJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/African_swine_fever_virus African swine fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAJ OCA].
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[[Category: Large Structures]]
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[[Category: Maciejewski MW]]
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==Reference==
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[[Category: Mullen GP]]
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Solution structure of a viral DNA repair polymerase., Maciejewski MW, Shin R, Pan B, Marintchev A, Denninger A, Mullen MA, Chen K, Gryk MR, Mullen GP, Nat Struct Biol. 2001 Nov;8(11):936-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11685238 11685238]
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[[Category: Pan B]]
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[[Category: African swine fever virus]]
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[[Category: Shin R]]
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[[Category: Single protein]]
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[[Category: Maciejewski, M W.]]
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[[Category: Mullen, G P.]]
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[[Category: Pan, B.]]
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[[Category: Shin, R.]]
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[[Category: cis peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:00:52 2008''
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Current revision

Solution Structure of DNA Polymerase X from the African Swine Fever Virus

PDB ID 1jaj

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