1jbj

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[[Image:1jbj.jpg|left|200px]]<br /><applet load="1jbj" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jbj" />
 
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'''CD3 Epsilon and gamma Ectodomain Fragment Complex in Single-Chain Construct'''<br />
 
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==Overview==
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==CD3 Epsilon and gamma Ectodomain Fragment Complex in Single-Chain Construct==
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The T cell receptor (TCR) consists of genetically diverse disulfide-linked alpha and beta chains in noncovalent association with the invariant CD3 subunits. CD3 epsilon and CD3 gamma are integral components of both the TCR and pre-TCR. Here, we present the solution structure of a heterodimeric CD3 epsilon gamma ectodomain complex. A unique side-to-side hydrophobic interface between the two C2-set immunoglobulin-like domains and parallel pairing of their respective C-terminal beta strands are revealed. Mutational analysis confirms the importance of the distinctive linkage as well as the membrane proximal stalk motif (RxCxxCxE) for domain-domain association. These biochemical and structural analyses offer insights into the modular pairwise association of CD3 invariant chains. More importantly, the findings suggest how the rigidified CD3 elements participate in TCR-based signal transduction.
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<StructureSection load='1jbj' size='340' side='right'caption='[[1jbj]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jbj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JBJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jbj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jbj OCA], [https://pdbe.org/1jbj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jbj RCSB], [https://www.ebi.ac.uk/pdbsum/1jbj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jbj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CD3E_MOUSE CD3E_MOUSE] The CD3 complex mediates signal transduction.[https://www.uniprot.org/uniprot/CD3G_MOUSE CD3G_MOUSE] Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G (PubMed:25920998).[UniProtKB:P09693]<ref>PMID:25920998</ref> <ref>PMID:9524111</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jb/1jbj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jbj ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JBJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBJ OCA].
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*[[CD3 3D structures|CD3 3D structures]]
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== References ==
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==Reference==
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<references/>
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Mechanisms contributing to T cell receptor signaling and assembly revealed by the solution structure of an ectodomain fragment of the CD3 epsilon gamma heterodimer., Sun ZJ, Kim KS, Wagner G, Reinherz EL, Cell. 2001 Jun 29;105(7):913-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11439187 11439187]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Kim KS]]
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[[Category: Kim, K S.]]
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[[Category: Reinherz EL]]
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[[Category: Reinherz, E L.]]
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[[Category: Sun Z-YJ]]
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[[Category: Sun, Z Y.J.]]
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[[Category: Wagner G]]
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[[Category: Wagner, G.]]
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[[Category: beta-sheet]]
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[[Category: c2-set immunoglobulin superfamily]]
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[[Category: h-bonded g strand pair]]
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[[Category: single-chain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:20:47 2008''
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CD3 Epsilon and gamma Ectodomain Fragment Complex in Single-Chain Construct

PDB ID 1jbj

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