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1jc9

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(New page: 200px<br /><applet load="1jc9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jc9, resolution 2.01&Aring;" /> '''TACHYLECTIN 5A FROM ...)
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[[Image:1jc9.jpg|left|200px]]<br /><applet load="1jc9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jc9, resolution 2.01&Aring;" />
 
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'''TACHYLECTIN 5A FROM TACHYPLEUS TRIDENTATUS (JAPANESE HORSESHOE CRAB)'''<br />
 
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==Overview==
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==TACHYLECTIN 5A FROM TACHYPLEUS TRIDENTATUS (JAPANESE HORSESHOE CRAB)==
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Because invertebrates lack an adaptive immune system, they had to evolve, effective intrinsic defense strategies against a variety of microbial, pathogens. This ancient form of host defense, the innate immunity, is, present in all multicellular organisms including humans. The innate immune, system of the Japanese horseshoe crab Tachypleus tridentatus, serving as a, model organism, includes a hemolymph coagulation system, which, participates both in defense against microbes and in hemostasis. Early, work on the evolution of vertebrate fibrinogen suggested a common origin, of the arthropod hemolymph coagulation and the vertebrate blood, coagulation systems. However, this conjecture could not be verified by, comparing the structures of coagulogen, the clotting protein of the, horseshoe crab, and of mammalian fibrinogen. Here we report the crystal, structure of tachylectin 5A (TL5A), a nonself-recognizing lectin from the, hemolymph plasma of T. tridentatus. TL5A shares not only a common fold but, also related functional sites with the gamma fragment of mammalian, fibrinogen. Our observations provide the first structural evidence of a, common ancestor for the innate immunity and the blood coagulation systems.
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<StructureSection load='1jc9' size='340' side='right'caption='[[1jc9]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1jc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tachypleus_tridentatus Tachypleus tridentatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JC9 FirstGlance]. <br>
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1JC9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Tachypleus_tridentatus Tachypleus tridentatus] with NAG and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JC9 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jc9 OCA], [https://pdbe.org/1jc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jc9 RCSB], [https://www.ebi.ac.uk/pdbsum/1jc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jc9 ProSAT]</span></td></tr>
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The 2.0-A crystal structure of tachylectin 5A provides evidence for the common origin of the innate immunity and the blood coagulation systems., Kairies N, Beisel HG, Fuentes-Prior P, Tsuda R, Muta T, Iwanaga S, Bode W, Huber R, Kawabata S, Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13519-24. Epub 2001 Nov 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11707569 11707569]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/TL5A_TACTR TL5A_TACTR] Lectin involved in innate immunity. Agglutinates all types of human erythrocytes, Gram-positive and Gram-negative bacteria. Has a stronger agglutinating activity towards Gram-negative bacteria than towards Gram-positive bacteria. Specifically recognizes acetyl group-containing substances on agglutinated cells. The hemagglutinating activity was inhibited by EDTA, acetyl group-containing mono- and disaccharides, N-acetyl derivatives of amino acids, other acetyl group-containing substances, propionamide and benzamide. Enhances the antimicrobial activity of big defensin against Gram-positive bacteria but not against Gram-negative bacteria.<ref>PMID:10468566</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jc/1jc9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jc9 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Tachypleus tridentatus]]
[[Category: Tachypleus tridentatus]]
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[[Category: Beisel, H.G.]]
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[[Category: Beisel H-G]]
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[[Category: Bode, W.]]
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[[Category: Bode W]]
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[[Category: Fuentes-Prior, P.]]
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[[Category: Fuentes-Prior P]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Iwanaga, S.]]
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[[Category: Iwanaga S]]
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[[Category: Kairies, N.]]
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[[Category: Kairies N]]
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[[Category: Kawabata, S.]]
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[[Category: Kawabata S]]
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[[Category: Muta, T.]]
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[[Category: Muta T]]
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[[Category: Tsuda, R.]]
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[[Category: Tsuda R]]
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[[Category: CA]]
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[[Category: NAG]]
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[[Category: fibrinogen related]]
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[[Category: lectin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:06:14 2007''
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Current revision

TACHYLECTIN 5A FROM TACHYPLEUS TRIDENTATUS (JAPANESE HORSESHOE CRAB)

PDB ID 1jc9

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