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1jf9

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(New page: 200px<br /><applet load="1jf9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jf9, resolution 2.&Aring;" /> '''Crystal Structure of s...)
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[[Image:1jf9.gif|left|200px]]<br /><applet load="1jf9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jf9, resolution 2.&Aring;" />
 
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'''Crystal Structure of selenocysteine lyase'''<br />
 
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==Overview==
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==Crystal Structure of selenocysteine lyase==
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The Escherichia coli NifS CsdB protein is a member of the homodimeric, pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes, are capable of decomposing cysteine or selenocysteine into L-alanine and, sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity, for L-selenocysteine in comparison to l-cysteine, suggesting a role for, this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli, NifS CsdB reveals a high-resolution view of the active site of this enzyme, in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and, perselenide intermediates during catalysis, a necessary intermediate in, the formation of sulfur and selenium containing metabolites.
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<StructureSection load='1jf9' size='340' side='right'caption='[[1jf9]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jf9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JF9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JF9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jf9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jf9 OCA], [https://pdbe.org/1jf9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jf9 RCSB], [https://www.ebi.ac.uk/pdbsum/1jf9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jf9 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1jf9 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SUFS_ECOLI SUFS_ECOLI] Cysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L-selenocysteine. Selenocysteine lyase activity is however unsure in vivo.<ref>PMID:10829016</ref> <ref>PMID:12089140</ref> <ref>PMID:11997471</ref> <ref>PMID:12876288</ref> <ref>PMID:12941942</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jf/1jf9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jf9 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JF9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PLP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Selenocysteine_lyase Selenocysteine lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.16 4.4.1.16] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JF9 OCA].
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*[[Selenocysteine lyase|Selenocysteine lyase]]
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== References ==
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==Reference==
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<references/>
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Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11827487 11827487]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Selenocysteine lyase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Burley SK]]
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[[Category: Burley, S.K.]]
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[[Category: Lima CD]]
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[[Category: Lima, C.D.]]
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[[Category: NYSGXRC, New.York.Structural.GenomiX.Research.Consortium.]]
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[[Category: PLP]]
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[[Category: cysteine]]
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[[Category: new york structural genomix research consortium]]
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[[Category: nifs]]
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[[Category: nysgxrc]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: pursulfide]]
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[[Category: selenocysteine]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:11:16 2007''
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Current revision

Crystal Structure of selenocysteine lyase

PDB ID 1jf9

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