This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1jkf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1jkf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jkf, resolution 2.4&Aring;" /> '''Holo 1L-myo-inositol-...)
Current revision (07:52, 3 April 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1jkf.gif|left|200px]]<br /><applet load="1jkf" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1jkf, resolution 2.4&Aring;" />
 
-
'''Holo 1L-myo-inositol-1-phosphate Synthase'''<br />
 
-
==Overview==
+
==Holo 1L-myo-inositol-1-phosphate Synthase==
-
1-l-myo-Inositol-1-phosphate synthase catalyzes the conversion of, d-glucose 6-phosphate to 1-l-myo-inositol-1-phosphate (MIP), the first and, rate-limiting step in the biosynthesis of all inositol-containing, compounds. It involves an oxidation, intramolecular aldol cyclization, and, reduction. We have determined the first crystal structure of MIP synthase., We present structures of both the NAD-bound enzyme and the enzyme bound to, an inhibitor, 2-deoxy-glucitol-6-phosphate. While 58 amino acids are, disordered in the unbound form of the enzyme in the vicinity of the active, site, the inhibitor nucleates the folding of this domain in a striking, example of induced fit, serving to completely encapsulate it within the, enzyme. Three helices and a long beta-strand are formed in this process., We postulate a mechanism for the conversion based on the structure of the, inhibitor-bound complex.
+
<StructureSection load='1jkf' size='340' side='right'caption='[[1jkf]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1jkf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JKF FirstGlance]. <br>
-
1JKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Inositol-3-phosphate_synthase Inositol-3-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.4 5.5.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JKF OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jkf OCA], [https://pdbe.org/1jkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jkf RCSB], [https://www.ebi.ac.uk/pdbsum/1jkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jkf ProSAT]</span></td></tr>
-
The crystal structure and mechanism of 1-L-myo-inositol- 1-phosphate synthase., Stein AJ, Geiger JH, J Biol Chem. 2002 Mar 15;277(11):9484-91. Epub 2002 Jan 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11779862 11779862]
+
</table>
-
[[Category: Inositol-3-phosphate synthase]]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/INO1_YEAST INO1_YEAST]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jk/1jkf_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jkf ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Single protein]]
+
[[Category: Geiger JH]]
-
[[Category: Geiger, J.H.]]
+
[[Category: Stein AJ]]
-
[[Category: Stein, A.J.]]
+
-
[[Category: NAD]]
+
-
[[Category: rossmann fold]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:20:40 2007''
+

Current revision

Holo 1L-myo-inositol-1-phosphate Synthase

PDB ID 1jkf

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jkf"
Personal tools