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1jr8

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(New page: 200px<br /><applet load="1jr8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jr8, resolution 1.5&Aring;" /> '''Crystal Structure of ...)
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[[Image:1jr8.jpg|left|200px]]<br /><applet load="1jr8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jr8, resolution 1.5&Aring;" />
 
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'''Crystal Structure of Erv2p'''<br />
 
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==Overview==
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==Crystal Structure of Erv2p==
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Erv2p is an FAD-dependent sulfhydryl oxidase that can promote disulfide, bond formation during protein biosynthesis in the yeast endoplasmic, reticulum. The structure of Erv2p, determined by X-ray crystallography to, 1.5 A resolution, reveals a helix-rich dimer with no global resemblance to, other known FAD-binding proteins or thiol oxidoreductases. Two pairs of, cysteine residues are required for Erv2p activity. The first, (Cys-Gly-Glu-Cys) is adjacent to the isoalloxazine ring of the FAD. The, second (Cys-Gly-Cys) is part of a flexible C-terminal segment that can, swing into the vicinity of the first cysteine pair in the opposite subunit, of the dimer and may shuttle electrons between substrate protein dithiols, and the FAD-proximal disulfide.
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<StructureSection load='1jr8' size='340' side='right'caption='[[1jr8]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jr8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JR8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jr8 OCA], [https://pdbe.org/1jr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jr8 RCSB], [https://www.ebi.ac.uk/pdbsum/1jr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jr8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ERV2_YEAST ERV2_YEAST] FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation in the endoplasmic reticulum lumen in parallel to ERO1.<ref>PMID:11313344</ref> <ref>PMID:11584268</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jr/1jr8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jr8 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JR8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with FAD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JR8 OCA].
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*[[Sulfhydryl oxidase 3D structures|Sulfhydryl oxidase 3D structures]]
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== References ==
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==Reference==
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<references/>
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A new FAD-binding fold and intersubunit disulfide shuttle in the thiol oxidase Erv2p., Gross E, Sevier CS, Vala A, Kaiser CA, Fass D, Nat Struct Biol. 2002 Jan;9(1):61-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11740506 11740506]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Fass D]]
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[[Category: Fass, D.]]
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[[Category: Gross E]]
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[[Category: Gross, E.]]
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[[Category: Kaiser CA]]
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[[Category: Kaiser, C.A.]]
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[[Category: Sevier CS]]
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[[Category: Sevier, C.S.]]
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[[Category: Vala A]]
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[[Category: Vala, A.]]
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[[Category: FAD]]
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[[Category: cxxc]]
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[[Category: fad]]
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[[Category: helical bundle]]
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[[Category: sulfhydryl oxidase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:30:44 2007''
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Current revision

Crystal Structure of Erv2p

PDB ID 1jr8

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