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1jrr

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(New page: 200px<br /> <applet load="1jrr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jrr, resolution 1.60&Aring;" /> '''HUMAN PLASMINOGEN A...)
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[[Image:1jrr.gif|left|200px]]<br />
 
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<applet load="1jrr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jrr, resolution 1.60&Aring;" />
 
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'''HUMAN PLASMINOGEN ACTIVATOR INHIBITOR-2.[LOOP (66-98) DELETIONMUTANT] COMPLEXED WITH PEPTIDE MIMIckING THE REACTIVE CENTER LOOP'''<br />
 
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==Overview==
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==HUMAN PLASMINOGEN ACTIVATOR INHIBITOR-2.[LOOP (66-98) DELETIONMUTANT] COMPLEXED WITH PEPTIDE MIMIckING THE REACTIVE CENTER LOOP==
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The structure of the serpin, plasminogen activator inhibitor type-2, (PAI-2), in a complex with a peptide mimicking its reactive center loop, (RCL) has been determined at 1.6-A resolution. The structure shows the, relaxed state serpin structure with a prominent six-stranded beta-sheet., Clear electron density is seen for all residues in the peptide. The P1, residue of the peptide binds to a well defined pocket at the base of PAI-2, that may be important in determining the specificity of protease, inhibition. The stressed-to-relaxed state (S --&gt; R) transition in PAI-2, can be modeled as the relative motion between a quasirigid core domain and, a smaller segment comprising helix hF and beta-strands s1A, s2A, and s3A., A comparison of the Ramachandran plots of the stressed and relaxed state, PAI-2 structures reveals the location of several hinge regions connecting, these two domains. The hinge regions cluster in three locations on the, structure, ensuring a cooperative S --&gt; R transition. We hypothesize that, the hinge formed by the conserved Gly(206) on beta-strand s3A in the, breach region of PAI-2 effects the S --&gt; R transition by altering its, backbone torsion angles. This torsional change is due to the binding of, the P14 threonine of the RCL to the open breach region of PAI-2.
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<StructureSection load='1jrr' size='340' side='right'caption='[[1jrr]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jrr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JRR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JRR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jrr OCA], [https://pdbe.org/1jrr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jrr RCSB], [https://www.ebi.ac.uk/pdbsum/1jrr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jrr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PAI2_HUMAN PAI2_HUMAN] Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jr/1jrr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jrr ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JRR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ACE and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JRR OCA].
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*[[Plasminogen activator inhibitor|Plasminogen activator inhibitor]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the complex of plasminogen activator inhibitor 2 with a peptide mimicking the reactive center loop., Jankova L, Harrop SJ, Saunders DN, Andrews JL, Bertram KC, Gould AR, Baker MS, Curmi PM, J Biol Chem. 2001 Nov 16;276(46):43374-82. Epub 2001 Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11546761 11546761]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Andrews, J.L.]]
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[[Category: Andrews JL]]
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[[Category: Baker, M.S.]]
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[[Category: Baker MS]]
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[[Category: Bertram, K.C.]]
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[[Category: Bertram KC]]
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[[Category: Curmi, P.M.G.]]
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[[Category: Curmi PMG]]
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[[Category: Gould, A.R.]]
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[[Category: Gould AR]]
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[[Category: Harrop, S.J.]]
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[[Category: Harrop SJ]]
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[[Category: Jankova, L.]]
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[[Category: Jankova L]]
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[[Category: Saunders, D.N.]]
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[[Category: Saunders DN]]
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[[Category: ACE]]
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[[Category: BME]]
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[[Category: peptide binding]]
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[[Category: serpin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:43:48 2007''
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Current revision

HUMAN PLASMINOGEN ACTIVATOR INHIBITOR-2.[LOOP (66-98) DELETIONMUTANT] COMPLEXED WITH PEPTIDE MIMIckING THE REACTIVE CENTER LOOP

PDB ID 1jrr

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