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1jrt

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(New page: 200px<br /><applet load="1jrt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jrt, resolution 1.7&Aring;" /> '''HEMIACETAL COMPLEX BE...)
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[[Image:1jrt.gif|left|200px]]<br /><applet load="1jrt" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jrt, resolution 1.7&Aring;" />
 
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'''HEMIACETAL COMPLEX BETWEEN LEUPEPTIN AND TRYPSIN'''<br />
 
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==Overview==
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==HEMIACETAL COMPLEX BETWEEN LEUPEPTIN AND TRYPSIN==
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Three-dimensional structures of trypsin with the reversible inhibitor, leupeptin have been determined in two different crystal forms. The first, structure was determined at 1.7 A resolution with R-factor = 17.7% in the, trigonal crystal space group P3(1)21, with unit cell dimensions of a = b =, 55.62 A, c = 110.51 A. The second structure was determined at a resolution, of 1.8 A with R-factor = 17.5% in the orthorhombic space group, P2(1)2(1)2(1), with unit cell dimensions of a = 63.69 A, b = 69.37 A, c =, 63.01 A. The overall protein structure is very similar in both crystal, forms, with RMS difference for main-chain atoms of 0.27 A. The leupeptin, backbone forms four hydrogen bonds with trypsin and a fifth hydrogen bond, interaction is mediated by a water molecule. The aldehyde carbonyl of, leupeptin forms a covalent bond of 1.42 A length with side-chain oxygen of, Ser-195 in the active site. The reaction of trypsin with leupeptin, proceeds through the formation of stable tetrahedral complex in which the, hemiacetal oxygen atom is pointing out of the oxyanion hole and forming a, hydrogen bond with His-57.
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<StructureSection load='1jrt' size='340' side='right'caption='[[1jrt]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jrt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JRT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AR7:AMINO{[(4S)-4-AMINO-5,5-DIHYDROXYPENTYL]AMINO}METHANIMINIUM'>AR7</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jrt OCA], [https://pdbe.org/1jrt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jrt RCSB], [https://www.ebi.ac.uk/pdbsum/1jrt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jrt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jr/1jrt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jrt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JRT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CA and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JRT OCA].
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*[[Trypsin 3D structures|Trypsin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Two crystal structures of the leupeptin-trypsin complex., Kurinov IV, Harrison RW, Protein Sci. 1996 Apr;5(4):752-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8845765 8845765]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Trypsin]]
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[[Category: Harrison RW]]
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[[Category: Harrison, R.W.]]
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[[Category: Kurinov IV]]
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[[Category: Kurinov, I.V.]]
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[[Category: ACE]]
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[[Category: CA]]
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[[Category: digestion]]
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[[Category: hydrolase]]
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[[Category: pancreas]]
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[[Category: serine protease]]
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[[Category: zymogen]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:31:40 2007''
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Current revision

HEMIACETAL COMPLEX BETWEEN LEUPEPTIN AND TRYPSIN

PDB ID 1jrt

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