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1js4

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[[Image:1js4.gif|left|200px]]<br /><applet load="1js4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1js4, resolution 2.0&Aring;" />
 
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'''ENDO/EXOCELLULASE:CELLOBIOSE FROM THERMOMONOSPORA'''<br />
 
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==Overview==
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==ENDO/EXOCELLULASE:CELLOBIOSE FROM THERMOMONOSPORA==
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Cellulase E4 from Thermomonospora fusca is unusual in that it has characteristics of both exo- and endo-cellulases. Here we report the crystal structure of a 68K M(r) fragment of E4 (E4-68) at 1.9 A resolution. E4-68 contains both a family 9 catalytic domain, exhibiting an (alpha/alpha)6 barrel fold, and a family III cellulose binding domain, having an antiparallel beta-sandwich fold. While neither of these folds is novel, E4-68 provides the first cellulase structure having interacting catalytic and cellulose binding domains. The complexes of E4-68 with cellopentaose, cellotriose and cellobiose reveal conformational changes associated with ligand binding and allow us to propose a catalytic mechanism for family 9 enzymes. We also provide evidence that E4 has two novel characteristics: first it combines exo- and endo-activities and second, when it functions as an exo-cellulase, it cleaves off cellotetraose units.
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<StructureSection load='1js4' size='340' side='right'caption='[[1js4]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1js4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JS4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PRD_900005:beta-cellobiose'>PRD_900005</scene>, <scene name='pdbligand=PRD_900021:beta-cellotriose'>PRD_900021</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1js4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1js4 OCA], [https://pdbe.org/1js4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1js4 RCSB], [https://www.ebi.ac.uk/pdbsum/1js4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1js4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GUN4_THEFU GUN4_THEFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/js/1js4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1js4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JS4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca] with <scene name='pdbligand=GLC:'>GLC</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Known structural/functional Sites: <scene name='pdbsite=AA1:Active+Site'>AA1</scene>, <scene name='pdbsite=AB1:Active+Site'>AB1</scene>, <scene name='pdbsite=CA1:Ca+Binding+Site'>CA1</scene>, <scene name='pdbsite=CA2:Ca+Binding+Site'>CA2</scene>, <scene name='pdbsite=CA3:Ca+Binding+Site'>CA3</scene> and <scene name='pdbsite=CA4:Ca+Binding+Site'>CA4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JS4 OCA].
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca., Sakon J, Irwin D, Wilson DB, Karplus PA, Nat Struct Biol. 1997 Oct;4(10):810-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9334746 9334746]
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[[Category: Large Structures]]
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[[Category: Cellulase]]
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[[Category: Single protein]]
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[[Category: Thermobifida fusca]]
[[Category: Thermobifida fusca]]
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[[Category: Karplus, P A.]]
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[[Category: Karplus PA]]
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[[Category: Sakon, J.]]
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[[Category: Sakon J]]
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[[Category: Wilson, D B.]]
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[[Category: Wilson DB]]
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[[Category: CA]]
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[[Category: GLC]]
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[[Category: glycosyl hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:26:12 2008''
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Current revision

ENDO/EXOCELLULASE:CELLOBIOSE FROM THERMOMONOSPORA

PDB ID 1js4

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