This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

1jus

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Crystal structure of the multidrug binding transcriptional repressor QacR bound to rhodamine 6G

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jus"

@@ Line 1: / Line 1: @@
-[[Image:1jus.jpg|left|200px]]<br /><applet load="1jus" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1jus, resolution 2.84&Aring;" />
-'''Crystal structure of the multidrug binding transcriptional repressor QacR bound to rhodamine 6G'''<br />
-==Overview==
+==Crystal structure of the multidrug binding transcriptional repressor QacR bound to rhodamine 6G==
-The Staphylococcus aureus multidrug binding protein QacR represses, transcription of the qacA multidrug transporter gene and is induced by, structurally diverse cationic lipophilic drugs. Here, we report the, crystal structures of six QacR-drug complexes. Compared to the DNA bound, structure, drug binding elicits a coil-to-helix transition that causes, induction and creates an expansive multidrug-binding pocket, containing, four glutamates and multiple aromatic and polar residues. These structures, indicate the presence of separate but linked drug-binding sites within a, single protein. This multisite drug-binding mechanism is consonant with, studies on multidrug resistance transporters.
+<StructureSection load='1jus' size='340' side='right'caption='[[1jus]], [[Resolution|resolution]] 2.84&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1jus]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JUS FirstGlance]. <br>
-JUS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with SO4 and RHQ as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JUS OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.84&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=RHQ:RHODAMINE+6G'>RHQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jus OCA], [https://pdbe.org/1jus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jus RCSB], [https://www.ebi.ac.uk/pdbsum/1jus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jus ProSAT]</span></td></tr>
-Structural mechanisms of QacR induction and multidrug recognition., Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG, Science. 2001 Dec 7;294(5549):2158-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11739955 11739955]
+</table>
-[[Category: Single protein]]
+== Function ==
+[https://www.uniprot.org/uniprot/QACR_STAAU QACR_STAAU] Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/1jus_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jus ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Staphylococcus aureus]]
-[[Category: Brennan, R.G.]]
+[[Category: Brennan RG]]
-[[Category: Brown, M.H.]]
+[[Category: Brown MH]]
-[[Category: Grkovic, S.]]
+[[Category: Grkovic S]]
-[[Category: Miller, M.C.]]
+[[Category: Miller MC]]
-[[Category: Schumacher, M.A.]]
+[[Category: Schumacher MA]]
-[[Category: Skurray, R.A.]]
+[[Category: Skurray RA]]
-[[Category: RHQ]]
-[[Category: SO4]]
-[[Category: cationic lipophilic drugs]]
-[[Category: multidrug recognition]]
-[[Category: qacr]]
-[[Category: rhodamine 6g]]
-[[Category: s. aureus]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:35:50 2007''

1jus

From Proteopedia

Current revision

Crystal structure of the multidrug binding transcriptional repressor QacR bound to rhodamine 6G

Structural highlights

Function

Evolutionary Conservation

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox