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1k23

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(New page: 200px<br /><applet load="1k23" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k23, resolution 3.00&Aring;" /> '''Inorganic Pyrophosph...)
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[[Image:1k23.jpg|left|200px]]<br /><applet load="1k23" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k23, resolution 3.00&Aring;" />
 
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'''Inorganic Pyrophosphatase (Family II) from Bacillus subtilis'''<br />
 
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==Overview==
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==Inorganic Pyrophosphatase (Family II) from Bacillus subtilis==
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Recently, a new class of soluble inorganic pyrophosphatase (type-C PPase), has been described that is not homologous in amino acid sequence or, kinetic properties to the well-studied PPases (types A and B) found in, many organisms from bacteria to humans and thought to be essential to the, cell. Structural studies of the type-C PPases from Streptococcus gordonii, and Bacillus subtilis reveal a homodimeric structure, with each, polypeptide folding into two domains joined by a flexible hinge. The, active site, formed at the interface between the N and C-terminal domains, binds two manganese ions approximately 3.6 A apart in a conformation, resembling binuclear metal centres found in other hydrolytic enzymes. An, activated water molecule bridging the two metal ions is likely poised for, nucleophilic attack of the substrate. Importantly, the S. gordonii and B., subtilis enzymes have crystallised in strikingly different conformations., In both subunits of the S. gordonii crystal structure (1.5 A resolution), the C-terminal domain is positioned such that the active site is occluded, with a sulphate ion bound in the active site. In contrast, in the B., subtilis structure (3.0 A resolution) the C-terminal domain is rotated by, about 90 degrees, leaving the active site wide open and accessible for, substrate binding.
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<StructureSection load='1k23' size='340' side='right'caption='[[1k23]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k23]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K23 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k23 OCA], [https://pdbe.org/1k23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k23 RCSB], [https://www.ebi.ac.uk/pdbsum/1k23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k23 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PPAC_BACSU PPAC_BACSU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/1k23_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k23 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1K23 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K23 OCA].
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*[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The "open" and "closed" structures of the type-C inorganic pyrophosphatases from Bacillus subtilis and Streptococcus gordonii., Ahn S, Milner AJ, Futterer K, Konopka M, Ilias M, Young TW, White SA, J Mol Biol. 2001 Nov 2;313(4):797-811. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11697905 11697905]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Inorganic diphosphatase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Ahn S]]
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[[Category: Ahn, S.]]
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[[Category: Futterer K]]
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[[Category: Futterer, K.]]
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[[Category: Ilias M]]
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[[Category: Ilias, M.]]
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[[Category: Konopka M]]
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[[Category: Konopka, M.]]
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[[Category: Milner AJ]]
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[[Category: Milner, A.J.]]
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[[Category: White SA]]
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[[Category: White, S.A.]]
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[[Category: Young TW]]
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[[Category: Young, T.W.]]
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[[Category: MN]]
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[[Category: binuclear metal centre]]
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[[Category: inorganic pyrophosphatase]]
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[[Category: manganese]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:47:19 2007''
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Current revision

Inorganic Pyrophosphatase (Family II) from Bacillus subtilis

PDB ID 1k23

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