1k28

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(New page: 200px<br /><applet load="1k28" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k28, resolution 2.9&Aring;" /> '''The Structure of the ...)
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[[Image:1k28.jpg|left|200px]]<br /><applet load="1k28" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k28, resolution 2.9&Aring;" />
 
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'''The Structure of the Bacteriophage T4 Cell-Puncturing Device'''<br />
 
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==Overview==
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==The Structure of the Bacteriophage T4 Cell-Puncturing Device==
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Bacteriophage T4 has a very efficient mechanism for infecting cells. The, key component of this process is the baseplate, located at the end of the, phage tail, which regulates the interaction of the tail fibres and the DNA, ejection machine. A complex of gene product (gp) 5 (63K) and gp27 (44K), the central part of the baseplate, is required to penetrate the outer cell, membrane of Escherichia coli and to disrupt the intermembrane, peptidoglycan layer, promoting subsequent entry of phage DNA into the, host. We present here a crystal structure of the (gp5-gp27)3 321K complex, determined to 2.9 A resolution and fitted into a cryo-electron microscopy, map at 17 A resolution of the baseplate-tail tube assembly. The, carboxy-terminal domain of gp5 is a triple-stranded beta-helix that forms, an equilateral triangular prism, which acts as a membrane-puncturing, needle. The middle lysozyme domain of gp5, situated on the periphery of, the prism, serves to digest the peptidoglycan layer. The amino-terminal, antiparallel beta-barrel domain of gp5 is inserted into a cylinder formed, by three gp27 monomers, which may serve as a channel for DNA ejection.
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<StructureSection load='1k28' size='340' side='right'caption='[[1k28]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k28]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K28 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k28 OCA], [https://pdbe.org/1k28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k28 RCSB], [https://www.ebi.ac.uk/pdbsum/1k28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k28 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NEEDL_BPT4 NEEDL_BPT4] Tail-associated lysozyme of the baseplate hub that is essential for localized hydrolysis of bacterial cell wall necessary for viral DNA injection. The needle-like gp5 protein punctures the outer cell membrane and then digests the peptidoglycan cell wall in the periplasmic space. Involved in the tail assembly.<ref>PMID:12837775</ref> <ref>PMID:21129200</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/1k28_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k28 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1K28 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with K and PO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K28 OCA].
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of the cell-puncturing device of bacteriophage T4., Kanamaru S, Leiman PG, Kostyuchenko VA, Chipman PR, Mesyanzhinov VV, Arisaka F, Rossmann MG, Nature. 2002 Jan 31;415(6871):553-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11823865 11823865]
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__TOC__
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[[Category: Bacteriophage t4]]
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</StructureSection>
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[[Category: Lysozyme]]
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[[Category: Escherichia virus T4]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Arisaka, F.]]
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[[Category: Arisaka F]]
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[[Category: Chipman, P.R.]]
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[[Category: Chipman PR]]
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[[Category: Kanamaru, S.]]
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[[Category: Kanamaru S]]
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[[Category: Kostyuchenko, V.A.]]
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[[Category: Kostyuchenko VA]]
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[[Category: Leiman, P.G.]]
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[[Category: Leiman PG]]
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[[Category: Mesyanzhinov, V.V.]]
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[[Category: Mesyanzhinov VV]]
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[[Category: Rossmann, M.G.]]
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[[Category: Rossmann MG]]
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[[Category: K]]
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[[Category: PO4]]
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[[Category: gp27-gp5*-gp5c]]
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[[Category: hub]]
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[[Category: ob fold]]
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[[Category: pseudohexamer]]
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[[Category: t4 tail lysozyme]]
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[[Category: triple-stranded beta-helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:47:24 2007''
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Current revision

The Structure of the Bacteriophage T4 Cell-Puncturing Device

PDB ID 1k28

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