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1k35

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(New page: 200px<br /><applet load="1k35" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k35, resolution 2.2&Aring;" /> '''Crystal Structure of ...)
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[[Image:1k35.gif|left|200px]]<br /><applet load="1k35" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k35, resolution 2.2&Aring;" />
 
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'''Crystal Structure of Phosphomannomutase/Phosphoglucomutase from P.aeruginosa'''<br />
 
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==Overview==
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==Crystal Structure of Phosphomannomutase/Phosphoglucomutase from P.aeruginosa==
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The enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) from P., aeruginosa is required for the biosynthesis of two bacterial, exopolysaccharides: alginate and lipopolysaccharide (LPS). Both of these, molecules play a role in the virulence of P. aeruginosa, an important, human pathogen known for its ability to develop antibiotic resistance and, cause chronic lung infections in cystic fibrosis patients. The crystal, structure of PMM/PGM shows that the enzyme has four domains, three of, which have a similar three-dimensional fold. Residues from all four, domains of the protein contribute to the formation of a large active site, cleft in the center of the molecule. Detailed information on the active, site of PMM/PGM lays the foundation for structure-based inhibitor design., Inhibitors of sufficient potency and specificity should impair the, biosynthesis of alginate and LPS, and may facilitate clearance of the, bacteria by the host immune system and increase the efficacy of, conventional antibiotic treatment against chronic P. aeruginosa, infections.
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<StructureSection load='1k35' size='340' side='right'caption='[[1k35]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k35]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K35 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k35 OCA], [https://pdbe.org/1k35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k35 RCSB], [https://www.ebi.ac.uk/pdbsum/1k35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k35 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALGC_PSEAE ALGC_PSEAE] The phosphomannomutase activity produces a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics. Also involved in core-LPS biosynthesis due to its phosphoglucomutase activity. Essential for rhamnolipid production, an exoproduct correlated with pathogenicity, and for biofilm production.<ref>PMID:7515870</ref> <ref>PMID:10481091</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k3/1k35_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k35 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1K35 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phosphomannomutase Phosphomannomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.8 5.4.2.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K35 OCA].
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*[[Phosphomannomutase|Phosphomannomutase]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of PMM/PGM: an enzyme in the biosynthetic pathway of P. aeruginosa virulence factors., Regni C, Tipton PA, Beamer LJ, Structure. 2002 Feb;10(2):269-79. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11839312 11839312]
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__TOC__
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[[Category: Phosphomannomutase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Beamer LJ]]
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[[Category: Beamer, L.J.]]
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[[Category: Regni C]]
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[[Category: Regni, C.]]
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[[Category: Tipton PA]]
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[[Category: Tipton, P.A.]]
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[[Category: ZN]]
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[[Category: alpha/beta protein]]
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[[Category: enzyme-metal complex]]
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[[Category: phosphoserine]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:48:47 2007''
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Current revision

Crystal Structure of Phosphomannomutase/Phosphoglucomutase from P.aeruginosa

PDB ID 1k35

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