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1kb9

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YEAST CYTOCHROME BC1 COMPLEX

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Retrieved from "http://52.214.119.220/wiki/index.php/1kb9"

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-[[Image:1kb9.gif|left|200px]]
- {{Structure
+==YEAST CYTOCHROME BC1 COMPLEX==
-|PDB= 1kb9 |SIZE=350|CAPTION= <scene name='initialview01'>1kb9</scene>, resolution 2.30&Aring;
+<StructureSection load='1kb9' size='340' side='right'caption='[[1kb9]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=SMA:STIGMATELLIN+A'>SMA</scene>, <scene name='pdbligand=UQ6:5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL'>UQ6</scene>, <scene name='pdbligand=PIE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOINOSITOL'>PIE</scene>, <scene name='pdbligand=PEF:DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE'>PEF</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=PCF:1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE'>PCF</scene> and <scene name='pdbligand=UMQ:UNDECYL-MALTOSIDE'>UMQ</scene>
+<table><tr><td colspan='2'>[[1kb9]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KB9 FirstGlance]. <br>
-|ACTIVITY= [http://en.wikipedia.org/wiki/Ubiquinol--cytochrome-c_reductase Ubiquinol--cytochrome-c reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.2.2 1.10.2.2]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PCF:1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE'>PCF</scene>, <scene name='pdbligand=PEF:DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE'>PEF</scene>, <scene name='pdbligand=PIE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOINOSITOL'>PIE</scene>, <scene name='pdbligand=SMA:STIGMATELLIN+A'>SMA</scene>, <scene name='pdbligand=UMQ:UNDECYL-MALTOSIDE'>UMQ</scene>, <scene name='pdbligand=UQ6:5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL'>UQ6</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kb9 OCA], [https://pdbe.org/1kb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kb9 RCSB], [https://www.ebi.ac.uk/pdbsum/1kb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kb9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CY1_YEAST CY1_YEAST] Heme-containing component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/1kb9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kb9 ConSurf].
+<div style="clear:both"></div>
-'''YEAST CYTOCHROME BC1 COMPLEX'''
+==See Also==
+*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
+*[[Cytochrome bc1 3D structures|Cytochrome bc1 3D structures]]
-==Overview==
+__TOC__
-Biochemical data have shown that specific, tightly bound phospholipids are essential for activity of the cytochrome bc1 complex (QCR), an integral membrane protein of the respiratory chain. However, the structure and function of such phospholipids are not yet known. Here we describe five phospholipid molecules and one detergent molecule in the X-ray structure of yeast QCR at 2.3 A resolution. Their individual binding sites suggest specific roles in facilitating structural and functional integrity of the enzyme. Interestingly, a phosphatidylinositol molecule is bound in an unusual interhelical position near the flexible linker region of the Rieske iron-sulfur protein. Two possible proton uptake pathways at the ubiquinone reduction site have been identified: the E/R and the CL/K pathway. Remarkably, cardiolipin is positioned at the entrance to the latter. We propose that cardiolipin ensures structural integrity of the proton-conducting protein environment and takes part directly in proton uptake. Site-directed mutagenesis of ligating residues confirmed the importance of the phosphatidylinositol- and cardiolipin-binding sites.
+</StructureSection>
+[[Category: Large Structures]]
-==About this Structure==
-KB9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KB9 OCA].
-==Reference==
-Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure., Lange C, Nett JH, Trumpower BL, Hunte C, EMBO J. 2001 Dec 3;20(23):6591-600. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11726495 11726495]
 [[Category: Mus musculus]]
-[[Category: Protein complex]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Ubiquinol--cytochrome-c reductase]]
+[[Category: Hunte C]]
-[[Category: Hunte, C.]]
+[[Category: Lange C]]
-[[Category: Lange, C.]]
+[[Category: Nett JH]]
-[[Category: Nett, J H.]]
+[[Category: Trumpower BL]]
-[[Category: Trumpower, B L.]]
-[[Category: CDL]]
-[[Category: FES]]
-[[Category: HEM]]
-[[Category: PCF]]
-[[Category: PEF]]
-[[Category: PIE]]
-[[Category: SMA]]
-[[Category: UMQ]]
-[[Category: UQ6]]
-[[Category: cardiolipin]]
-[[Category: oxidoreductase]]
-[[Category: phosphatidylcholin]]
-[[Category: phosphatidylethanolamin]]
-[[Category: phosphatidylinositol]]
-[[Category: stigmatellin]]
-[[Category: ubiquinone]]
-[[Category: undecyl-maltopyranoside]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:15:00 2008''

1kb9

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YEAST CYTOCHROME BC1 COMPLEX

Structural highlights

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Evolutionary Conservation

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