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1kbe

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[[Image:1kbe.jpg|left|200px]]
 
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==Solution structure of the cysteine-rich C1 domain of Kinase Suppressor of Ras==
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The line below this paragraph, containing "STRUCTURE_1kbe", creates the "Structure Box" on the page.
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<StructureSection load='1kbe' size='340' side='right'caption='[[1kbe]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1kbe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KBE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KBE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1kbe| PDB=1kbe | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kbe OCA], [https://pdbe.org/1kbe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kbe RCSB], [https://www.ebi.ac.uk/pdbsum/1kbe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kbe ProSAT]</span></td></tr>
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</table>
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'''Solution structure of the cysteine-rich C1 domain of Kinase Suppressor of Ras'''
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== Function ==
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[https://www.uniprot.org/uniprot/KSR1_MOUSE KSR1_MOUSE] Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Kinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that acts as a molecular scaffold to promote signal transmission from Raf-1 to MEK and MAPK. All KSR proteins contain a conserved cysteine-rich C1 domain, and studies have implicated this domain in the regulation of KSR1 subcellular localization and function. To further elucidate the biological role of the KSR1 C1 domain, we have determined its three-dimensional solution structure using nuclear magnetic resonance (NMR). We find that while the overall topology of the KSR1 C1 domain is similar to the C1 domains of Raf-1 and PKCgamma, the predicted ligand-binding region and the surface charge distribution are unique. Moreover, by generating chimeric proteins in which these domains have been swapped, we find that the C1 domains of Raf-1, PKCgamma, and KSR1 are not functionally interchangeable. The KSR1 C1 domain does not bind with high affinity or respond biologically to phorbol esters or ceramide, and it does not interact directly with Ras, indicating that the putative ligand(s) for the KSR1 C1 domain are distinct from those that interact with PKCgamma and Raf-1. In addition, our analysis of the chimeric proteins supports the model that Raf-1 is a ceramide-activated kinase and that its C1 domain is involved in the ceramide-mediated response. Finally, our findings demonstrate an absolute requirement of the KSR1 C1 domain in mediating the membrane localization of KSR1, a crucial feature of its scaffolding activity. Together, these results underscore the functional specificity of these important regulatory domains and demonstrate that the structural features of the C1 domains can provide valuable insight into their ligand-binding properties.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/1kbe_consurf.spt"</scriptWhenChecked>
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1KBE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KBE OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Solution structure and functional analysis of the cysteine-rich C1 domain of kinase suppressor of Ras (KSR)., Zhou M, Horita DA, Waugh DS, Byrd RA, Morrison DK, J Mol Biol. 2002 Jan 18;315(3):435-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11786023 11786023]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kbe ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Byrd RA]]
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[[Category: Byrd, R A.]]
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[[Category: Horita DA]]
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[[Category: Horita, D A.]]
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[[Category: Morrison DK]]
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[[Category: Morrison, D K.]]
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[[Category: Waugh DS]]
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[[Category: Waugh, D S.]]
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[[Category: Zhou M]]
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[[Category: Zhou, M.]]
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[[Category: Cysteine-rich domain]]
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[[Category: Kinase suppressor of ra]]
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[[Category: Ksr]]
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[[Category: Zinc-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:32:11 2008''
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Current revision

Solution structure of the cysteine-rich C1 domain of Kinase Suppressor of Ras

PDB ID 1kbe

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