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1kdg

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(New page: 200px<br /><applet load="1kdg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kdg, resolution 1.50&Aring;" /> '''Crystal structure of...)
Current revision (07:59, 3 April 2024) (edit) (undo)
 
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[[Image:1kdg.gif|left|200px]]<br /><applet load="1kdg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kdg, resolution 1.50&Aring;" />
 
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'''Crystal structure of the flavin domain of cellobiose dehydrogenase'''<br />
 
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==Overview==
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==Crystal structure of the flavin domain of cellobiose dehydrogenase==
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Cellobiose dehydrogenase (CDH) participates in the degradation of, cellulose and lignin. The protein is an extracellular flavocytochrome with, a b-type cytochrome domain (CYT(cdh)) connected to a flavodehydrogenase, domain (DH(cdh)). DH(cdh) catalyses a two-electron oxidation at the, anomeric C1 position of cellobiose to yield cellobiono-1,5-lactone, and, the electrons are subsequently transferred from DH(cdh) to an acceptor, either directly or via CYT(cdh). Here, we describe the crystal structure, of Phanerochaete chrysosporium DH(cdh) determined at 1.5 A resolution., DH(cdh) belongs to the GMC family of oxidoreductases, which includes, glucose oxidase (GOX) and cholesterol oxidase (COX); however, the sequence, identity with members of the family is low. The overall fold of DH(cdh) is, p-hydroxybenzoate hydroxylase-like and is similar to, but also different, from, that of GOX and COX. It is partitioned into an FAD-binding subdomain, of alpha/beta type and a substrate-binding subdomain consisting of a, seven-stranded beta sheet and six helices. Docking of CYT(cdh) and DH(cdh), suggests that CYT(cdh) covers the active-site entrance in DH(cdh), and, that the resulting distance between the cofactors is within acceptable, limits for inter-domain electron transfer. Based on docking of the, substrate, cellobiose, in the active site of DH(cdh), we propose that the, enzyme discriminates against glucose by favouring interaction with the, non-reducing end of cellobiose.
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<StructureSection load='1kdg' size='340' side='right'caption='[[1kdg]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1kdg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phanerodontia_chrysosporium Phanerodontia chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KDG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KDG FirstGlance]. <br>
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1KDG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium] with NAG, MAN, HG, 6FA, EMT and S as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KDG OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6FA:6-HYDROXY-FLAVIN-ADENINE+DINUCLEOTIDE'>6FA</scene>, <scene name='pdbligand=EMT:2-(ETHYLMERCURI-THIO)-BENZOIC+ACID'>EMT</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kdg OCA], [https://pdbe.org/1kdg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kdg RCSB], [https://www.ebi.ac.uk/pdbsum/1kdg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kdg ProSAT]</span></td></tr>
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Crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase., Hallberg BM, Henriksson G, Pettersson G, Divne C, J Mol Biol. 2002 Jan 18;315(3):421-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11786022 11786022]
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</table>
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[[Category: Phanerochaete chrysosporium]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/CDH_PHACH CDH_PHACH] Degrades both lignin and cellulose. Oxidizes cellobiose to cellobionolactone.
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[[Category: Divne, C.]]
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== Evolutionary Conservation ==
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[[Category: Hallberg, B.M.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Henriksson, G.]]
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Check<jmol>
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[[Category: Pettersson, G.]]
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<jmolCheckbox>
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[[Category: 6FA]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kd/1kdg_consurf.spt"</scriptWhenChecked>
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[[Category: EMT]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: HG]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: MAN]]
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</jmolCheckbox>
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[[Category: NAG]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kdg ConSurf].
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[[Category: S]]
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<div style="clear:both"></div>
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[[Category: 6-hydroxylated fad]]
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__TOC__
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[[Category: alpha/beta structure]]
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</StructureSection>
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[[Category: gmc oxidoreductase]]
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[[Category: Large Structures]]
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[[Category: phbh fold]]
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[[Category: Phanerodontia chrysosporium]]
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[[Category: rossmann fold]]
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[[Category: Divne C]]
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[[Category: Hallberg BM]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:04:57 2007''
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[[Category: Henriksson G]]
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[[Category: Pettersson G]]

Current revision

Crystal structure of the flavin domain of cellobiose dehydrogenase

PDB ID 1kdg

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