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1kdm

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(New page: 200px<br /> <applet load="1kdm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kdm, resolution 2.35&Aring;" /> '''THE CRYSTAL STRUCTU...)
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[[Image:1kdm.gif|left|200px]]<br />
 
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<applet load="1kdm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kdm, resolution 2.35&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF THE HUMAN SEX HORMONE-BINDING GLOBULIN (TETRAGONAL CRYSTAL FORM)'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF THE HUMAN SEX HORMONE-BINDING GLOBULIN (TETRAGONAL CRYSTAL FORM)==
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The crystal structure of human sex hormone-binding globulin (SHBG) has, revealed how 5alpha-dihydrotestosterone intercalates between the two, seven-stranded beta-sheets of its amino-terminal laminin G-like domain., However, a region of disorder (residues 130 to 135 of SHBG) was identified, together with a zinc-binding site in immediate proximity to the steroid., It has been important to resolve the structure of this region because, previous studies have suggested that these residues may contribute to, steroid binding directly. Here, we present the 2.35 A and 1.7 A crystal, structures of the amino-terminal LG domain of SHBG obtained from a, tetragonal crystal form and by EDTA-soaking of a trigonal crystal form, respectively. In both of these new structures, residues Pro130 to Arg135, are now clearly visible. Substitution of the two residues (Leu131Gly and, Lys134Ala) pointing towards the steroid has shown that only Leu131, contributes significantly to steroid binding. Rather than covering the, steroid-binding pocket in an extended conformation, a 3(10) helical turn, is formed by residues Leu131 to Lys134 in this segment. Unfolding of this, secondary structure element can either facilitate the entry of the, steroids into the binding site or modulate the important contribution that, Leu131 makes to steroid binding. A comparison with previous structures, supports the concept that zinc binding re-orients the side-chain of, His136, and this residue serves as a lever causing disorder within the, loop structure between Pro130 and Arg135.
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<StructureSection load='1kdm' size='340' side='right'caption='[[1kdm]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1kdm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KDM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KDM FirstGlance]. <br>
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1KDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and DHT as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KDM OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DHT:5-ALPHA-DIHYDROTESTOSTERONE'>DHT</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kdm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kdm OCA], [https://pdbe.org/1kdm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kdm RCSB], [https://www.ebi.ac.uk/pdbsum/1kdm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kdm ProSAT]</span></td></tr>
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Resolution of a disordered region at the entrance of the human sex hormone-binding globulin steroid-binding site., Grishkovskaya I, Avvakumov GV, Hammond GL, Muller YA, J Mol Biol. 2002 May 3;318(3):621-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12054810 12054810]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SHBG_HUMAN SHBG_HUMAN] Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kd/1kdm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kdm ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Avvakumov, G.V.]]
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[[Category: Avvakumov GV]]
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[[Category: Grishkovskaya, I.]]
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[[Category: Grishkovskaya I]]
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[[Category: Hammond, G.L.]]
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[[Category: Hammond GL]]
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[[Category: Muller, Y.A.]]
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[[Category: Muller YA]]
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[[Category: CA]]
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[[Category: DHT]]
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[[Category: dht]]
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[[Category: shbg]]
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[[Category: tetragonal crystal form]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:50:08 2007''
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Current revision

THE CRYSTAL STRUCTURE OF THE HUMAN SEX HORMONE-BINDING GLOBULIN (TETRAGONAL CRYSTAL FORM)

PDB ID 1kdm

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