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1kem

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(New page: 200px<br /> <applet load="1kem" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kem, resolution 2.20&Aring;" /> '''CATALYTIC ANTIBODY ...)
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[[Image:1kem.gif|left|200px]]<br />
 
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<applet load="1kem" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kem, resolution 2.20&Aring;" />
 
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'''CATALYTIC ANTIBODY 28B4 FAB FRAGMENT'''<br />
 
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==Overview==
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==CATALYTIC ANTIBODY 28B4 FAB FRAGMENT==
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The x-ray crystal structures of the sulfide oxidase antibody 28B4 and of, antibody 28B4 complexed with hapten have been solved at 2.2-angstrom and, 1.9-angstrom resolution, respectively. To our knowledge, these structures, are the highest resolution catalytic antibody structures to date and, provide insight into the molecular mechanism of this antibody-catalyzed, monooxygenation reaction. Specifically, the data suggest that entropic, restriction plays a fundamental role in catalysis through the precise, alignment of the thioether substrate and oxidant. The antibody active site, also stabilizes developing charge on both sulfur and periodate in the, transition state via cation-pi and electrostatic interactions, respectively. In addition to demonstrating that the active site of, antibody 28B4 does indeed reflect the mechanistic information programmed, in the aminophosphonic acid hapten, these high-resolution structures, provide a basis for enhancing turnover rates through mutagenesis and, improved hapten design.
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<StructureSection load='1kem' size='340' side='right'caption='[[1kem]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kem]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KEM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kem OCA], [https://pdbe.org/1kem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kem RCSB], [https://www.ebi.ac.uk/pdbsum/1kem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kem ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IGHG1_MOUSE IGHG1_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ke/1kem_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kem ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KEM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KEM OCA].
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Insights into antibody catalysis: structure of an oxygenation catalyst at 1.9-angstrom resolution., Hsieh-Wilson LC, Schultz PG, Stevens RC, Proc Natl Acad Sci U S A. 1996 May 28;93(11):5363-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8643580 8643580]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Hsieh-Wilson LC]]
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[[Category: Hsieh-Wilson, L.C.]]
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[[Category: Schultz PG]]
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[[Category: Schultz, P.G.]]
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[[Category: Stevens RC]]
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[[Category: Stevens, R.C.]]
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[[Category: catalytic antibody]]
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[[Category: fab]]
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[[Category: immunoglobulin]]
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[[Category: monooxygenase]]
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[[Category: oxygenation]]
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[[Category: sulfide oxidation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:34:54 2007''
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Current revision

CATALYTIC ANTIBODY 28B4 FAB FRAGMENT

PDB ID 1kem

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