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1kll

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[[Image:1kll.jpg|left|200px]]
 
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==Molecular basis of mitomycin C resictance in streptomyces: Crystal structures of the MRD protein with and without a drug derivative==
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The line below this paragraph, containing "STRUCTURE_1kll", creates the "Structure Box" on the page.
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<StructureSection load='1kll' size='340' side='right'caption='[[1kll]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1kll]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lavendulae Streptomyces lavendulae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KLL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KLL FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MC:1,2-CIS-1-HYDROXY-2,7-DIAMINO-MITOSENE'>MC</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_1kll| PDB=1kll | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kll OCA], [https://pdbe.org/1kll PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kll RCSB], [https://www.ebi.ac.uk/pdbsum/1kll PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kll ProSAT]</span></td></tr>
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</table>
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'''MOLECULAR BASIS OF MITOMYCIN C RESICTANCE IN STREPTOMYCES: CRYSTAL STRUCTURES OF THE MRD PROTEIN WITH AND WITHOUT A DRUG DERIVATIVE'''
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== Function ==
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[https://www.uniprot.org/uniprot/O05205_STRLA O05205_STRLA]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Mitomycin C (MC) is a potent anticancer agent. Streptomyces lavendulae, which produces MC, protects itself from the lethal effects of the drug by expressing several resistance proteins. One of them (MRD) binds MC and functions as a drug exporter. We report the crystal structure of MRD and its complex with an MC metabolite, 1,2-cis-1-hydroxy-2,7-diaminomitosene, at 1.5 A resolution. The drug is sandwiched by pi-stacking interactions of His-38 and Trp-108. MRD is a dimer. The betaalphabetabetabeta fold of the MRD molecule is reminiscent of methylmalonyl-CoA epimerase, bleomycin resistance proteins, glyoxalase I, and extradiol dioxygenases. The location of the binding site is identical to the ones in evolutionarily related enzymes, suggesting that the protein may have been recruited from a different metabolic pathway.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/1kll_consurf.spt"</scriptWhenChecked>
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1KLL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_lavendulae Streptomyces lavendulae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KLL OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Molecular basis of mitomycin C resistance in streptomyces: structure and function of the MRD protein., Martin TW, Dauter Z, Devedjiev Y, Sheffield P, Jelen F, He M, Sherman DH, Otlewski J, Derewenda ZS, Derewenda U, Structure. 2002 Jul;10(7):933-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12121648 12121648]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kll ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces lavendulae]]
[[Category: Streptomyces lavendulae]]
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[[Category: Dauter, Z.]]
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[[Category: Dauter Z]]
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[[Category: Derewenda, U.]]
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[[Category: Derewenda U]]
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[[Category: Derewenda, Z S.]]
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[[Category: Derewenda ZS]]
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[[Category: Devedjiev, Y.]]
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[[Category: Devedjiev Y]]
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[[Category: He, M.]]
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[[Category: He M]]
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[[Category: Jelen, F.]]
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[[Category: Jelen F]]
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[[Category: Martin, T W.]]
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[[Category: Martin TW]]
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[[Category: Otlewski, J.]]
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[[Category: Otlewski J]]
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[[Category: Sheffield, P.]]
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[[Category: Sheffield P]]
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[[Category: Sherman, D.]]
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[[Category: Sherman D]]
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[[Category: Anomalous diffraction]]
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[[Category: Antibiotic resistance]]
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[[Category: Crystal structure]]
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[[Category: Domain swapping]]
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[[Category: Mitomycin c]]
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[[Category: P-staking]]
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[[Category: Sad]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:53:07 2008''
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Current revision

Molecular basis of mitomycin C resictance in streptomyces: Crystal structures of the MRD protein with and without a drug derivative

PDB ID 1kll

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