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1kng

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(New page: 200px<br /><applet load="1kng" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kng, resolution 1.14&Aring;" /> '''Crystal structure of...)
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[[Image:1kng.gif|left|200px]]<br /><applet load="1kng" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kng, resolution 1.14&Aring;" />
 
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'''Crystal structure of CcmG reducing oxidoreductase at 1.14 A'''<br />
 
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==Overview==
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==Crystal structure of CcmG reducing oxidoreductase at 1.14 A==
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CcmG is unlike other periplasmic thioredoxin (TRX)-like proteins in that, it has a specific reducing activity in an oxidizing environment and a high, fidelity of interaction. These two unusual properties are required for its, role in c-type cytochrome maturation. The crystal structure of CcmG, reveals a modified TRX fold with an unusually acidic active site and a, groove formed from two inserts in the fold. Deletion of one of the, groove-forming inserts disrupts c-type cytochrome formation. Two unique, structural features of CcmG-an acidic active site and an adjacent, groove-appear to be necessary to convert an indiscriminately binding, scaffold, the TRX fold, into a highly specific redox protein.
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<StructureSection load='1kng' size='340' side='right'caption='[[1kng]], [[Resolution|resolution]] 1.14&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kng]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KNG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.14&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kng FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kng OCA], [https://pdbe.org/1kng PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kng RCSB], [https://www.ebi.ac.uk/pdbsum/1kng PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kng ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYCY_BRADU CYCY_BRADU] Required for disulfide bond formation in some periplasmic proteins. Also acts as a disulfide oxidoreductase in cytochromes c biogenesis. The cysteines of apocytochromes c must be in the reduced state for covalent linkage between the two moieties to occur (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kn/1kng_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kng ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KNG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KNG OCA].
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*[[Thiol:disulfide interchange protein 3D structures|Thiol:disulfide interchange protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of CcmG/DsbE at 1.14 A resolution: high-fidelity reducing activity in an indiscriminately oxidizing environment., Edeling MA, Guddat LW, Fabianek RA, Thony-Meyer L, Martin JL, Structure. 2002 Jul;10(7):973-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12121652 12121652]
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[[Category: Bradyrhizobium japonicum]]
[[Category: Bradyrhizobium japonicum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Edeling, M.A.]]
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[[Category: Edeling MA]]
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[[Category: Fabianek, R.A.]]
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[[Category: Fabianek RA]]
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[[Category: Guddat, L.W.]]
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[[Category: Guddat LW]]
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[[Category: Martin, J.L.]]
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[[Category: Martin JL]]
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[[Category: Thony-Meyer, L.]]
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[[Category: Thony-Meyer L]]
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[[Category: atomic resolution]]
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[[Category: cytochrome c maturation]]
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[[Category: thioredoxin fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:24:59 2007''
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Current revision

Crystal structure of CcmG reducing oxidoreductase at 1.14 A

PDB ID 1kng

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