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1kob

From Proteopedia

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[[Image:1kob.gif|left|200px]]<br /><applet load="1kob" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kob, resolution 2.3&Aring;" />
 
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'''TWITCHIN KINASE FRAGMENT (APLYSIA), AUTOREGULATED PROTEIN KINASE DOMAIN'''<br />
 
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==Overview==
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==TWITCHIN KINASE FRAGMENT (APLYSIA), AUTOREGULATED PROTEIN KINASE DOMAIN==
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The myosin-associated giant protein kinases twitchin and titin are composed predominantly of fibronectin- and immunoglobulin-like modules. We report the crystal structures of two autoinhibited twitchin kinase fragments, one from Aplysia and a larger fragment from Caenorhabditis elegans containing an additional C-terminal immunoglobulin-like domain. The structure of the longer fragment shows that the immunoglobulin domain contacts the protein kinase domain on the opposite side from the catalytic cleft, laterally exposing potential myosin binding residues. Together, the structures reveal the cooperative interactions between the autoregulatory region and the residues from the catalytic domain involved in protein substrate binding, ATP binding, catalysis and the activation loop, and explain the differences between the observed autoinhibitory mechanism and the one found in the structure of calmodulin-dependent kinase I.
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<StructureSection load='1kob' size='340' side='right'caption='[[1kob]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1kob]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KOB FirstGlance]. <br>
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1KOB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica] with <scene name='pdbligand=VAL:'>VAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOB OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VAL:VALINE'>VAL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kob OCA], [https://pdbe.org/1kob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kob RCSB], [https://www.ebi.ac.uk/pdbsum/1kob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kob ProSAT]</span></td></tr>
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Giant protein kinases: domain interactions and structural basis of autoregulation., Kobe B, Heierhorst J, Feil SC, Parker MW, Benian GM, Weiss KR, Kemp BE, EMBO J. 1996 Dec 16;15(24):6810-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9003756 9003756]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q16980_APLCA Q16980_APLCA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/1kob_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kob ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Aplysia californica]]
[[Category: Aplysia californica]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Benian, G M.]]
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[[Category: Benian GM]]
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[[Category: Feil, S C.]]
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[[Category: Feil SC]]
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[[Category: Heierhorst, J.]]
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[[Category: Heierhorst J]]
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[[Category: Kemp, B E.]]
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[[Category: Kemp BE]]
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[[Category: Kobe, B.]]
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[[Category: Kobe B]]
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[[Category: Parker, M W.]]
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[[Category: Parker MW]]
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[[Category: Weiss, K R.]]
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[[Category: Weiss KR]]
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[[Category: VAL]]
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[[Category: intrasteric regulation]]
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[[Category: kinase]]
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[[Category: twitchin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:14 2008''
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Current revision

TWITCHIN KINASE FRAGMENT (APLYSIA), AUTOREGULATED PROTEIN KINASE DOMAIN

PDB ID 1kob

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