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1ksi

From Proteopedia

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[[Image:1ksi.gif|left|200px]]<br />
 
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<applet load="1ksi" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ksi, resolution 2.2&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A EUKARYOTIC (PEA SEEDLING) COPPER-CONTAINING AMINE OXIDASE AT 2.2A RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A EUKARYOTIC (PEA SEEDLING) COPPER-CONTAINING AMINE OXIDASE AT 2.2A RESOLUTION==
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BACKGROUND: Copper-containing amine oxidases catalyze the oxidative, deamination of primary amines to aldehydes, in a reaction that requires, free radicals. These enzymes are important in many biological processes, including cell differentiation and growth, would healing, detoxification, and signalling. The catalytic reaction requires a redox cofactor, topa, quinone (TPQ), which is derived by post-translational modification of an, invariant tyrosine residue. Both the biogenesis of the TPQ cofactor and, the reaction catalyzed by the enzyme require the presence of a copper atom, at the active site. The crystal structure of a prokaryotic copper amine, oxidase from E. coli (ECAO) has recently been reported. RESULTS: The first, structure of a eukaryotic (pea seedling) amine oxidase (PSAO) has ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8805580 (full description)]]
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<StructureSection load='1ksi' size='340' side='right'caption='[[1ksi]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ksi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KSI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ksi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ksi OCA], [https://pdbe.org/1ksi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ksi RCSB], [https://www.ebi.ac.uk/pdbsum/1ksi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ksi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMO_PEA AMO_PEA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/1ksi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ksi ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KSI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pivum_sativum Pivum sativum]] with NAG, CU and MN as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6]]. Structure known Active Sites: ACA and ACB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KSI OCA]].
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*[[Copper amine oxidase 3D structures|Copper amine oxidase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of a eukaryotic (pea seedling) copper-containing amine oxidase at 2.2 A resolution., Kumar V, Dooley DM, Freeman HC, Guss JM, Harvey I, McGuirl MA, Wilce MC, Zubak VM, Structure. 1996 Aug 15;4(8):943-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8805580 8805580]
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[[Category: Large Structures]]
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[[Category: Amine oxidase (copper-containing)]]
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[[Category: Pisum sativum]]
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[[Category: Pivum sativum]]
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[[Category: Freeman HC]]
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[[Category: Single protein]]
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[[Category: Guss JM]]
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[[Category: Freeman, H.C.]]
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[[Category: Kumar V]]
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[[Category: Guss, J.M.]]
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[[Category: Wilce MCJ]]
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[[Category: Kumar, V.]]
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[[Category: Wilce, M.C.J.]]
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[[Category: CU]]
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[[Category: MN]]
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[[Category: NAG]]
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[[Category: copper]]
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[[Category: oxidase]]
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[[Category: oxidoreductase]]
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[[Category: pea seedling]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:41:39 2007''
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Current revision

CRYSTAL STRUCTURE OF A EUKARYOTIC (PEA SEEDLING) COPPER-CONTAINING AMINE OXIDASE AT 2.2A RESOLUTION

PDB ID 1ksi

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