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1kyq

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[[Image:1kyq.gif|left|200px]]
 
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{{Structure
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==Met8p: A bifunctional NAD-dependent dehydrogenase and ferrochelatase involved in siroheme synthesis.==
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|PDB= 1kyq |SIZE=350|CAPTION= <scene name='initialview01'>1kyq</scene>, resolution 2.2&Aring;
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<StructureSection load='1kyq' size='340' side='right'caption='[[1kyq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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<table><tr><td colspan='2'>[[1kyq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KYQ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE= Met8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kyq OCA], [https://pdbe.org/1kyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kyq RCSB], [https://www.ebi.ac.uk/pdbsum/1kyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kyq ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kyq OCA], [http://www.ebi.ac.uk/pdbsum/1kyq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kyq RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/MET8_YEAST MET8_YEAST] Catalyzes the conversion of precorrin-2 into siroheme. This reaction consist of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme.<ref>PMID:10051442</ref>
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== Evolutionary Conservation ==
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'''Met8p: A bifunctional NAD-dependent dehydrogenase and ferrochelatase involved in siroheme synthesis.'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ky/1kyq_consurf.spt"</scriptWhenChecked>
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Sirohaem is a tetrapyrrole-derived prosthetic group that is required for the essential assimilation of sulfur and nitrogen into all living systems as part of the sulfite and nitrite reductase systems. The final two steps in the biosynthesis of sirohaem involve a beta-NAD(+)-dependent dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by ferrochelation to yield sirohaem. In Saccharomyces cerevisiae, Met8p is a bifunctional enzyme that carries out both of these reactions. Here, we report the 2.2 A resolution crystal structure of Met8p, which adopts a novel fold that bears no resemblance to the previously determined structures of cobalt- or ferro-chelatases. Analysis of mutant proteins suggests that both catalytic activities share a single active site, and that Asp141 plays an essential role in both dehydrogenase and chelatase processes.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1KYQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KYQ OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kyq ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase., Schubert HL, Raux E, Brindley AA, Leech HK, Wilson KS, Hill CP, Warren MJ, EMBO J. 2002 May 1;21(9):2068-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11980703 11980703]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Brindley AA]]
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[[Category: Brindley, A A.]]
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[[Category: Hill CP]]
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[[Category: Hill, C P.]]
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[[Category: Raux E]]
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[[Category: Raux, E.]]
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[[Category: Schubert HL]]
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[[Category: Schubert, H L.]]
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[[Category: Warren MJ]]
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[[Category: Warren, M J.]]
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[[Category: Wilson KS]]
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[[Category: Wilson, K S.]]
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[[Category: homodimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:55:30 2008''
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Current revision

Met8p: A bifunctional NAD-dependent dehydrogenase and ferrochelatase involved in siroheme synthesis.

PDB ID 1kyq

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