This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

1kyq

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Met8p: A bifunctional NAD-dependent dehydrogenase and ferrochelatase involved in siroheme synthesis.

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kyq"

@@ Line 1: / Line 1: @@
-[[Image:1kyq.gif|left|200px]]<br /><applet load="1kyq" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1kyq, resolution 2.2&Aring;" />
-'''Met8p: A bifunctional NAD-dependent dehydrogenase and ferrochelatase involved in siroheme synthesis.'''<br />
-==Overview==
+==Met8p: A bifunctional NAD-dependent dehydrogenase and ferrochelatase involved in siroheme synthesis.==
-Sirohaem is a tetrapyrrole-derived prosthetic group that is required for, the essential assimilation of sulfur and nitrogen into all living systems, as part of the sulfite and nitrite reductase systems. The final two steps, in the biosynthesis of sirohaem involve a beta-NAD(+)-dependent, dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by, ferrochelation to yield sirohaem. In Saccharomyces cerevisiae, Met8p is a, bifunctional enzyme that carries out both of these reactions. Here, we, report the 2.2 A resolution crystal structure of Met8p, which adopts a, novel fold that bears no resemblance to the previously determined, structures of cobalt- or ferro-chelatases. Analysis of mutant proteins, suggests that both catalytic activities share a single active site, and, that Asp141 plays an essential role in both dehydrogenase and chelatase, processes.
+<StructureSection load='1kyq' size='340' side='right'caption='[[1kyq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1kyq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KYQ FirstGlance]. <br>
-KYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KYQ OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kyq OCA], [https://pdbe.org/1kyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kyq RCSB], [https://www.ebi.ac.uk/pdbsum/1kyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kyq ProSAT]</span></td></tr>
-The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase., Schubert HL, Raux E, Brindley AA, Leech HK, Wilson KS, Hill CP, Warren MJ, EMBO J. 2002 May 1;21(9):2068-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11980703 11980703]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MET8_YEAST MET8_YEAST] Catalyzes the conversion of precorrin-2 into siroheme. This reaction consist of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme.<ref>PMID:10051442</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ky/1kyq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kyq ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Single protein]]
+[[Category: Brindley AA]]
-[[Category: Brindley, A.A.]]
+[[Category: Hill CP]]
-[[Category: Hill, C.P.]]
+[[Category: Raux E]]
-[[Category: Raux, E.]]
+[[Category: Schubert HL]]
-[[Category: Schubert, H.L.]]
+[[Category: Warren MJ]]
-[[Category: Warren, M.J.]]
+[[Category: Wilson KS]]
-[[Category: Wilson, K.S.]]
-[[Category: NAD]]
-[[Category: homodimer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:04:22 2007''

1kyq

From Proteopedia

Current revision

Met8p: A bifunctional NAD-dependent dehydrogenase and ferrochelatase involved in siroheme synthesis.

Structural highlights

Function

Evolutionary Conservation

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox