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1kz7

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[[Image:1kz7.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of the DH/PH Fragment of Murine Dbs in Complex with the Placental Isoform of Human Cdc42==
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|PDB= 1kz7 |SIZE=350|CAPTION= <scene name='initialview01'>1kz7</scene>, resolution 2.40&Aring;
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<StructureSection load='1kz7' size='340' side='right'caption='[[1kz7]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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<table><tr><td colspan='2'>[[1kz7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KZ7 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE= Dbs ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), Cdc42 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kz7 OCA], [https://pdbe.org/1kz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kz7 RCSB], [https://www.ebi.ac.uk/pdbsum/1kz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kz7 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1foe|1FOE]], [[1kzg|1KZG]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kz7 OCA], [http://www.ebi.ac.uk/pdbsum/1kz7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kz7 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/CDC42_HUMAN CDC42_HUMAN] Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.<ref>PMID:14978216</ref> <ref>PMID:15642749</ref> <ref>PMID:17038317</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kz/1kz7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kz7 ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of the DH/PH Fragment of Murine Dbs in Complex with the Placental Isoform of Human Cdc42'''
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==See Also==
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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== References ==
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==Overview==
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<references/>
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Dbl-related oncoproteins are guanine nucleotide exchange factors (GEFs) specific for Rho guanosine triphosphatases (GTPases) and invariably possess tandem Dbl (DH) and pleckstrin homology (PH) domains. While it is known that the DH domain is the principal catalytic subunit, recent biochemical data indicate that for some Dbl-family proteins, such as Dbs and Trio, PH domains may cooperate with their associated DH domains in promoting guanine nucleotide exchange of Rho GTPases. In order to gain an understanding of the involvement of these PH domains in guanine nucleotide exchange, we have determined the crystal structure of a DH/PH fragment from Dbs in complex with Cdc42. The complex features the PH domain in a unique conformation distinct from the PH domains in the related structures of Sos1 and Tiam1.Rac1. Consequently, the Dbs PH domain participates with the DH domain in binding Cdc42, primarily through a set of interactions involving switch 2 of the GTPase. Comparative sequence analysis suggests that a subset of Dbl-family proteins will utilize their PH domains similarly to Dbs.
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__TOC__
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</StructureSection>
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==About this Structure==
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1KZ7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KZ7 OCA].
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==Reference==
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A crystallographic view of interactions between Dbs and Cdc42: PH domain-assisted guanine nucleotide exchange., Rossman KL, Worthylake DK, Snyder JT, Siderovski DP, Campbell SL, Sondek J, EMBO J. 2002 Mar 15;21(6):1315-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11889037 11889037]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Campbell SL]]
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[[Category: Campbell, S L.]]
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[[Category: Rossman KL]]
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[[Category: Rossman, K L.]]
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[[Category: Siderovski DP]]
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[[Category: Siderovski, D P.]]
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[[Category: Snyder JT]]
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[[Category: Snyder, J T.]]
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[[Category: Sondek J]]
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[[Category: Sondek, J.]]
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[[Category: Worthylake DK]]
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[[Category: Worthylake, D K.]]
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[[Category: guanine nucleotide exchange factor (gef)]]
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[[Category: small g-protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:55:46 2008''
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Current revision

Crystal Structure of the DH/PH Fragment of Murine Dbs in Complex with the Placental Isoform of Human Cdc42

PDB ID 1kz7

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