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1l3h

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(New page: 200px<br /> <applet load="1l3h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l3h" /> '''NMR structure of P41icf, a potent inhibitor...)
Current revision (08:05, 3 April 2024) (edit) (undo)
 
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[[Image:1l3h.gif|left|200px]]<br />
 
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<applet load="1l3h" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1l3h" />
 
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'''NMR structure of P41icf, a potent inhibitor of human cathepsin L'''<br />
 
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==Overview==
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==NMR structure of P41icf, a potent inhibitor of human cathepsin L==
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The total synthesis and structural characterization of the, MHCII-associated p41 invariant chain fragment (P41icf) is described., P41icf plays a crucial role in the maturation of MHC class II molecules, and antigen processing, acting as a highly selective cathepsin L, inhibitor. P41icf synthesis was achieved using a combined, solid-phase/solution approach. The entire molecule (65 residues, 7246 Da, unprotected) was assembled in solution from fully protected peptides in, the size range of 10 residues. After deprotection, oxidative folding in, carefully adjusted experimental conditions led to the completely folded, and functional P41icf with a disulfide pairing identical to that of native, P41icf. CD, NMR, and surface plasmon resonance (SPR) were used for the, structural and functional characterization of synthetic P41icf. CD thermal, denaturation showed clear cooperative behavior. Tight cathepsin L binding, was demonstrated by SPR. (1)H NMR spectroscopy at 800 MHz of unlabeled, P41icf was used to solve the three-dimensional structure of the molecule., P41icf behaves as a well-folded protein domain with a topology very close, to the crystallographic cathepsin L-bound form.
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<StructureSection load='1l3h' size='340' side='right'caption='[[1l3h]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1l3h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L3H FirstGlance]. <br>
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1L3H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L3H OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l3h OCA], [https://pdbe.org/1l3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l3h RCSB], [https://www.ebi.ac.uk/pdbsum/1l3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l3h ProSAT]</span></td></tr>
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==Reference==
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</table>
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Synthesis and NMR structure of p41icf, a potent inhibitor of human cathepsin L., Chiva C, Barthe P, Codina A, Gairi M, Molina F, Granier C, Pugniere M, Inui T, Nishio H, Nishiuchi Y, Kimura T, Sakakibara S, Albericio F, Giralt E, J Am Chem Soc. 2003 Feb 12;125(6):1508-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12568610 12568610]
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== Disease ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/HG2A_HUMAN HG2A_HUMAN] Note=A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74-ROS1 chimeric protein.<ref>PMID:12661006</ref>
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[[Category: Barthe, P.]]
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== Function ==
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[[Category: Chiva, C.]]
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[https://www.uniprot.org/uniprot/HG2A_HUMAN HG2A_HUMAN] Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
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[[Category: Codina, A.]]
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== Evolutionary Conservation ==
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[[Category: Giralt, E.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: alpha helix]]
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Check<jmol>
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[[Category: beta sheet]]
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<jmolCheckbox>
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[[Category: disulfide bonds]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l3/1l3h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:56:41 2007''
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l3h ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Barthe P]]
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[[Category: Chiva C]]
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[[Category: Codina A]]
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[[Category: Giralt E]]

Current revision

NMR structure of P41icf, a potent inhibitor of human cathepsin L

PDB ID 1l3h

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