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1l3x

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(New page: 200px<br /><applet load="1l3x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l3x" /> '''Solution Structure of Novel Disintegrin Salm...)
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[[Image:1l3x.gif|left|200px]]<br /><applet load="1l3x" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1l3x" />
 
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'''Solution Structure of Novel Disintegrin Salmosin'''<br />
 
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==Overview==
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==Solution Structure of Novel Disintegrin Salmosin==
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Disintegrins are potent inhibitors of both platelet aggregation and, integrin-dependent cell adhesion. A new disintegrin, salmosin, isolated, from the venom of the Korean snake Agkistrodon halys brevicaudus, has been, characterized by mass spectrometry and NMR spectroscopy, and its in vitro, biological activity has been assessed. The IC(50) value of the purified, salmosin was determined to be 2.2 nM in an assay for the inhibition of, glycoprotein IIb-IIIa/fibrinogen interaction. Salmosin also inhibited the, bovine capillary endothelial cell proliferation induced by bFGF in a, dose-dependent manner. The NMR solution structures were well converged, with a root-mean-square deviation of 0.76 A for backbone atoms among the, 20 lowest energy structures, except for the arginylglycylaspartic acid, (RGD) loop. The structure revealed that the conserved RGD motif with an, unusual finger shape is distal from the rigid core of the C-terminal, domain. Furthermore, even though the RGD motif did not interact with the, hydrophobic core of the protein, it was stabilized by a network of, molecular contacts through a small antiparallel beta-sheet comprising, residues of Ile46-Ala50 and Asp54-Tyr58. Last, the electrostatic charge, distribution on the surface of salmosin differs dramatically from that of, other disintegrin proteins in that there is a cluster of negatively, charged residues in close proximity to the RGD loop.
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<StructureSection load='1l3x' size='340' side='right'caption='[[1l3x]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1l3x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gloydius_brevicaudus Gloydius brevicaudus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1iq2 1iq2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L3X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l3x OCA], [https://pdbe.org/1l3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l3x RCSB], [https://www.ebi.ac.uk/pdbsum/1l3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l3x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VM2HS_GLOBR VM2HS_GLOBR] Metalloproteinase hxl-1: snake venom metalloproteinase that impairs hemostasis in the envenomed animal (By similarity).<ref>PMID:9722022</ref> <ref>PMID:9856345</ref> <ref>PMID:10446992</ref> <ref>PMID:10944460</ref> <ref>PMID:14977354</ref> Disintegrin salmosin-1: inhibits GPIIb/GPIIIa (ITGA2B/ITGB3) binding to immobilized fibrinogen with an IC(50) of 2.2 nM and ADP-induced platelet aggregation with an IC(50) of 131 nM, respectively. Inhibits angiogenesis.<ref>PMID:9722022</ref> <ref>PMID:9856345</ref> <ref>PMID:10446992</ref> <ref>PMID:10944460</ref> <ref>PMID:14977354</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l3/1l3x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l3x ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1L3X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gloydius_blomhoffi_brevicaudus Gloydius blomhoffi brevicaudus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L3X OCA].
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*[[Disintegrin|Disintegrin]]
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== References ==
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==Reference==
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<references/>
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Solution structure of a novel disintegrin, salmosin, from Agkistrondon halys venom., Shin J, Hong SY, Chung K, Kang I, Jang Y, Kim DS, Lee W, Biochemistry. 2003 Dec 16;42(49):14408-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14661951 14661951]
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__TOC__
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[[Category: Gloydius blomhoffi brevicaudus]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Gloydius brevicaudus]]
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[[Category: Lee, W.]]
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[[Category: Large Structures]]
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[[Category: Shin, J.]]
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[[Category: Lee W]]
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[[Category: disintegrin]]
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[[Category: Shin J]]
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[[Category: rgd]]
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[[Category: snake venome]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:27:48 2007''
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Current revision

Solution Structure of Novel Disintegrin Salmosin

PDB ID 1l3x

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