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1l5e

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[[Image:1l5e.jpg|left|200px]]
 
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{{Structure
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==The domain-swapped dimer of CV-N in solution==
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|PDB= 1l5e |SIZE=350|CAPTION= <scene name='initialview01'>1l5e</scene>
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<StructureSection load='1l5e' size='340' side='right'caption='[[1l5e]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1l5e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L5E FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5e OCA], [https://pdbe.org/1l5e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l5e RCSB], [https://www.ebi.ac.uk/pdbsum/1l5e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5e ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[2ezm|2EZM]], [[3ezm|3EZM]], [[1l5b|1L5B]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5e OCA], [http://www.ebi.ac.uk/pdbsum/1l5e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1l5e RCSB]</span>
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[https://www.uniprot.org/uniprot/CVN_NOSEL CVN_NOSEL] Mannose-binding lectin.<ref>PMID:9210678</ref> <ref>PMID:12678493</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''The domain-swapped dimer of CV-N in solution'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l5/1l5e_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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The structure of the potent HIV-inactivating protein cyanovirin-N was previously found by NMR to be a monomer in solution and a domain-swapped dimer by X-ray crystallography. Here we demonstrate that, in solution, CV-N can exist both in monomeric and in domain-swapped dimeric form. The dimer is a metastable, kinetically trapped structure at neutral pH and room temperature. Based on orientational NMR constraints, we show that the domain-swapped solution dimer is similar to structures in two different crystal forms, exhibiting solely a small reorientation around the hinge region. Mutation of the single proline residue in the hinge to glycine significantly stabilizes the protein in both its monomeric and dimeric forms. By contrast, mutation of the neighboring serine to proline results in an exclusively dimeric protein, caused by a drastic destabilization of the monomer.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l5e ConSurf].
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1L5E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5E OCA].
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<div style="clear:both"></div>
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== References ==
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==Reference==
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<references/>
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The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures., Barrientos LG, Louis JM, Botos I, Mori T, Han Z, O'Keefe BR, Boyd MR, Wlodawer A, Gronenborn AM, Structure. 2002 May;10(5):673-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12015150 12015150]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Nostoc ellipsosporum]]
[[Category: Nostoc ellipsosporum]]
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[[Category: Single protein]]
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[[Category: Barrientos LG]]
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[[Category: Barrientos, L G.]]
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[[Category: Botos I]]
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[[Category: Botos, I.]]
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[[Category: Boyd MR]]
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[[Category: Boyd, M R.]]
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[[Category: Gronenborn AM]]
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[[Category: Gronenborn, A M.]]
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[[Category: Han Z]]
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[[Category: Han, Z.]]
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[[Category: Louis JM]]
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[[Category: Keefe, B R.O.]]
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[[Category: Mori T]]
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[[Category: Louis, J M.]]
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[[Category: O'Keefe BR]]
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[[Category: Mori, T.]]
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[[Category: Wlodawer A]]
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[[Category: Wlodawer, A.]]
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[[Category: 3d domain-swapping]]
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[[Category: cyanovirin-n]]
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[[Category: nmr]]
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[[Category: protein folding]]
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[[Category: x-ray]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:58:17 2008''
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Current revision

The domain-swapped dimer of CV-N in solution

PDB ID 1l5e

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