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1l8f

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(New page: 200px<br /><applet load="1l8f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l8f, resolution 1.8&Aring;" /> '''Structure of 20K-endo...)
Current revision (08:07, 3 April 2024) (edit) (undo)
 
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[[Image:1l8f.jpg|left|200px]]<br /><applet load="1l8f" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1l8f, resolution 1.8&Aring;" />
 
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'''Structure of 20K-endoglucanase from Melanocarpus albomyces at 1.8 A'''<br />
 
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==Overview==
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==Structure of 20K-endoglucanase from Melanocarpus albomyces at 1.8 A==
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The crystal structure of the 20K endoglucanase from the thermophilic, fungus Melanocarpus albomyces (Ma20k) has been determined. The structure, was refined to 1.8 A resolution using data obtained at 120 K. Ma20k, belongs to glycoside hydrolase family 45. The three-dimensional structures, of endoglucanase V (EGV) from the fungus Humicola insolens and of an, endoglucanase from H. grisea var. thermoidea have previously been, determined. The overall structure of Ma20k consists of a six-stranded, beta-barrel domain similar to that found previously in family 45, endoglucanases. The flexible loop between strands V and VI, which was, disordered in the uncomplexed structures of the Humicola endoglucanases, but was ordered in complexed structures of EGV, is found to be well, ordered in the native structure of Ma20k. The structure of Ma20k allows, comparison between thermophilic and mesophilic proteins of family 45 and, different principles for thermostability are discussed.
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<StructureSection load='1l8f' size='340' side='right'caption='[[1l8f]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1l8f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L8F FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l8f OCA], [https://pdbe.org/1l8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l8f RCSB], [https://www.ebi.ac.uk/pdbsum/1l8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l8f ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8J0K8_MELAO Q8J0K8_MELAO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l8/1l8f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l8f ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1L8F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L8F OCA].
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of 20K endoglucanase from Melanocarpus albomyces at 1.8 A resolution., Valjakka J, Rouvinen J, Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):765-8. Epub 2003, Mar 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12657806 12657806]
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[[Category: Large Structures]]
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[[Category: Cellulase]]
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[[Category: Melanocarpus albomyces]]
[[Category: Melanocarpus albomyces]]
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[[Category: Single protein]]
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[[Category: Rouvinen J]]
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[[Category: Rouvinen, J.]]
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[[Category: Valjakka J]]
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[[Category: Valjakka, J.]]
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[[Category: cellulase]]
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[[Category: endoglucanase]]
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[[Category: thermophilic]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:38:13 2007''
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Current revision

Structure of 20K-endoglucanase from Melanocarpus albomyces at 1.8 A

PDB ID 1l8f

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