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1lia

From Proteopedia

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(New page: 200px<br /><applet load="1lia" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lia, resolution 2.8&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1lia.gif|left|200px]]<br /><applet load="1lia" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lia, resolution 2.8&Aring;" />
 
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'''CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN FROM POLYSIPHONIA AT 2.8 A RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN FROM POLYSIPHONIA AT 2.8 A RESOLUTION==
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The structure of R-phycoerythrin (R-PE) from Polysiphonia urceolata was, determined at 2.8 A resolution. The crystals belong to space group R3 with, unit cell dimensions of a = b = 189.8 A, c = 60.1 A. The subunit, composition of R-PE is (alpha 2 beta 2)3 gamma. The three-dimensional, structure of R-PE was solved by the multiple isomorphous replacement, method. After several cycles of model building and refinement, the, crystallographic R-factor of the final model is 18.0% with data from 10.0, to 2.8 A resolution. The four phycoerythrobilin chromophores alpha 84, alpha 140a, beta 84 and beta 155 in an (alpha beta) unit are each, covalently bound to a cysteine residue through ring A. The phycourobilin, chromophore is bound to cysteine beta 50 by ring A and bound to cysteine, beta 61 by ring D. The ring A and ring D of phycourobilin deviate from the, conjugate plane formed by ring B and ring C and the four rings form a, boat-shaped structure. R-PE contains a 34 kDa gamma subunit that is, assumed to lie in the central channel of the molecular disc (alpha 2 beta, 2)3. The energy transfer and relationship between cysteine residues and, chromophores are discussed.
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<StructureSection load='1lia' size='340' side='right'caption='[[1lia]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1lia]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Polysiphonia_urceolata Polysiphonia urceolata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LIA FirstGlance]. <br>
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1LIA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Polysiphonia_urceolata Polysiphonia urceolata] with CYC and PUB as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LIA OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=PUB:PHYCOUROBILIN'>PUB</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lia OCA], [https://pdbe.org/1lia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lia RCSB], [https://www.ebi.ac.uk/pdbsum/1lia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lia ProSAT]</span></td></tr>
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Crystal structure of R-phycoerythrin from Polysiphonia urceolata at 2.8 A resolution., Chang WR, Jiang T, Wan ZL, Zhang JP, Yang ZX, Liang DC, J Mol Biol. 1996 Oct 11;262(5):721-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8876649 8876649]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHEA_POLUR PHEA_POLUR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/li/1lia_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lia ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Polysiphonia urceolata]]
[[Category: Polysiphonia urceolata]]
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[[Category: Protein complex]]
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[[Category: Chang WR]]
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[[Category: Chang, W.R.]]
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[[Category: Jiang T]]
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[[Category: Jiang, T.]]
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[[Category: Liang DC]]
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[[Category: Liang, D.C.]]
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[[Category: CYC]]
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[[Category: PUB]]
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[[Category: light harvesting protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:34:39 2007''
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Current revision

CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN FROM POLYSIPHONIA AT 2.8 A RESOLUTION

PDB ID 1lia

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