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1lmz

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[[Image:1lmz.gif|left|200px]]
 
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{{Structure
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==Solution Structure of 3-Methyladenine DNA Glycosylase I (TAG)==
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|PDB= 1lmz |SIZE=350|CAPTION= <scene name='initialview01'>1lmz</scene>
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<StructureSection load='1lmz' size='340' side='right'caption='[[1lmz]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1lmz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_B Escherichia coli B]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LMZ FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_I DNA-3-methyladenine glycosylase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.20 3.2.2.20]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE= TAG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lmz OCA], [https://pdbe.org/1lmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lmz RCSB], [https://www.ebi.ac.uk/pdbsum/1lmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lmz ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/3MG1_ECOLI 3MG1_ECOLI] Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lm/1lmz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lmz ConSurf].
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<div style="clear:both"></div>
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'''Solution Structure of 3-Methyladenine DNA Glycosylase I (TAG)'''
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==See Also==
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*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The Escherichia coli enzyme 3-methyladenine DNA glycosylase I (TAG) hydrolyzes the glycosidic bond of 3-methyladenine (3-MeA) in DNA and is found in many bacteria and some higher eukaryotes. TAG shows little primary sequence identity with members of the well-known helix-hairpin-helix (HhH) superfamily of DNA repair glycosylases, which consists of AlkA, EndoIII, MutY and hOGG1. Unexpectedly, the three-dimensional solution structure reported here reveals TAG as member of this superfamily. The restricted specificity of TAG for 3-MeA bases probably arises from its unique aromatic rich 3-MeA binding pocket and the absence of a catalytic aspartate that is present in all other HhH family members.
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[[Category: Escherichia coli B]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Drohat AC]]
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1LMZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMZ OCA].
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[[Category: Krosky DJ]]
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[[Category: Kwon K]]
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==Reference==
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[[Category: Stivers JT]]
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3-Methyladenine DNA glycosylase I is an unexpected helix-hairpin-helix superfamily member., Drohat AC, Kwon K, Krosky DJ, Stivers JT, Nat Struct Biol. 2002 Sep;9(9):659-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12161745 12161745]
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[[Category: DNA-3-methyladenine glycosylase I]]
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[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Drohat, A C.]]
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[[Category: Krosky, D J.]]
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[[Category: Kwon, K.]]
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[[Category: Stivers, J T.]]
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[[Category: 3-methyladenine]]
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[[Category: dna glycosylase]]
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[[Category: enzyme]]
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[[Category: helix-hairpin-helix superfamily]]
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[[Category: nmr spectroscopy]]
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[[Category: solution structure]]
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[[Category: tag]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:32:43 2008''
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Current revision

Solution Structure of 3-Methyladenine DNA Glycosylase I (TAG)

PDB ID 1lmz

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