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1lnl

From Proteopedia

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[[Image:1lnl.gif|left|200px]]<br /><applet load="1lnl" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lnl, resolution 3.30&Aring;" />
 
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'''Structure of deoxygenated hemocyanin from Rapana thomasiana'''<br />
 
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==Overview==
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==Structure of deoxygenated hemocyanin from Rapana thomasiana==
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Structure-function relationships in a molluscan hemocyanin have been investigated by determining the crystal structure of the Rapana thomasiana (gastropod) hemocyanin functional unit RtH2e in deoxygenated form at 3.38 A resolution. This is the first X-ray structure of an unit from the wall of the molluscan hemocyanin cylinder. The crystal structure of RtH2e demonstrates molecular self-assembly of six identical molecules forming a regular hexameric cylinder. This suggests how the functional units are ordered in the wall of the native molluscan hemocyanins. The molecular arrangement is stabilized by specific protomer-to-protomer interactions, which are probably typical for the functional units building the wall of the cylinders. A molecular mechanism for cooperative dioxygen binding in molluscan hemocyanins is proposed on the basis of the molecular interactions between the protomers. In particular, the deoxygenated RtH2e structure reveals a tunnel leading from two opposite sides of the molecule to the active site. The tunnel represents a possible entrance pathway for dioxygen molecules. No such tunnels have been observed in the crystal structure of the oxy-Odg, a functional unit from the Octopus dofleini (cephalopod) hemocyanin in oxygenated form.
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<StructureSection load='1lnl' size='340' side='right'caption='[[1lnl]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1lnl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rapana_venosa Rapana venosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LNL FirstGlance]. <br>
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1LNL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rapana_thomasiana Rapana thomasiana] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNL OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lnl OCA], [https://pdbe.org/1lnl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lnl RCSB], [https://www.ebi.ac.uk/pdbsum/1lnl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lnl ProSAT]</span></td></tr>
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The structure of a functional unit from the wall of a gastropod hemocyanin offers a possible mechanism for cooperativity., Perbandt M, Guthohrlein EW, Rypniewski W, Idakieva K, Stoeva S, Voelter W, Genov N, Betzel C, Biochemistry. 2003 Jun 3;42(21):6341-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12767214 12767214]
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</table>
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[[Category: Rapana thomasiana]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/HCY2E_RAPVE HCY2E_RAPVE] Hemocyanins are copper-containing oxygen carriers occurring freely dissolved in the hemolymph of many mollusks and arthropods.[UniProtKB:P12659]
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[[Category: Betzel, C.]]
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== Evolutionary Conservation ==
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[[Category: Genov, N.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Guthoehrlein, E W.]]
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Check<jmol>
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[[Category: Idakieva, K.]]
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<jmolCheckbox>
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[[Category: Perbandt, M.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ln/1lnl_consurf.spt"</scriptWhenChecked>
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[[Category: Rypniewski, W.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Stoeva, S.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Voelter, W.]]
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</jmolCheckbox>
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[[Category: CU]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lnl ConSurf].
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[[Category: NAG]]
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<div style="clear:both"></div>
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[[Category: cooperativity]]
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__TOC__
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[[Category: copper]]
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</StructureSection>
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[[Category: deoxygenated form]]
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[[Category: Large Structures]]
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[[Category: hemocyanin]]
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[[Category: Rapana venosa]]
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[[Category: metal binding site]]
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[[Category: Betzel C]]
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[[Category: oxygen]]
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[[Category: Genov N]]
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[[Category: Guthoehrlein EW]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:34 2008''
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[[Category: Idakieva K]]
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[[Category: Perbandt M]]
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[[Category: Rypniewski W]]
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[[Category: Stoeva S]]
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[[Category: Voelter W]]

Current revision

Structure of deoxygenated hemocyanin from Rapana thomasiana

PDB ID 1lnl

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