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1lnz

From Proteopedia

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[[Image:1lnz.gif|left|200px]]<br /><applet load="1lnz" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lnz, resolution 2.60&Aring;" />
 
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'''Structure of the Obg GTP-binding protein'''<br />
 
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==Overview==
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==Structure of the Obg GTP-binding protein==
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The Obg nucleotide binding protein family has been implicated in stress response, chromosome partitioning, replication initiation, mycelium development, and sporulation. Obg proteins are among a large group of GTP binding proteins conserved from bacteria to man. Members of the family contain two equally and highly conserved domains, a C-terminal GTP binding domain and an N-terminal glycine-rich domain. Structural analysis of Bacillus subtilis Obg revealed respective domain architectures and how they are coupled through the putative switch elements of the C-terminal GTPase domain in apo and nucleotide-bound configurations. Biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active sight suggest a potential role for ppGpp modulation of Obg function in B. subtilis.
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<StructureSection load='1lnz' size='340' side='right'caption='[[1lnz]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lnz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LNZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G4P:GUANOSINE-5,3-TETRAPHOSPHATE'>G4P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lnz OCA], [https://pdbe.org/1lnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lnz RCSB], [https://www.ebi.ac.uk/pdbsum/1lnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lnz ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1lnz TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OBG_BACSU OBG_BACSU] Necessary for the transition from vegetative growth to stage 0 or stage II of sporulation, but sporulation subsequent to these stages is unaffected at 45 degrees Celsius. This ts effect is probably due solely to the E-79 mutation. Required for expression of early sporulation genes, further suggesting a role in the induction of sporulation. Depletion effects on sporulation can be partially suppressed by missense mutations in spo0A. Strains depleted for obg stop growing after about 3 hours and do not induce the sigma-B factor following ethanol stress. It cofractionates with the ribosome and upstream stress response regulators RsbR, RsbS and RsbT in size fractionation columns, suggesting the ribosome might serve as a possible mediator of the activity of obg and the stress induction of sigma-B. In glycerol gradients partially associates with ribosomes; this is stabilized by a nonhydrolyzable GTP-analog and to a lesser extent GTP and GDP.<ref>PMID:7768831</ref> <ref>PMID:10419966</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ln/1lnz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lnz ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LNZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=G4P:'>G4P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNZ OCA].
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structural and biochemical analysis of the Obg GTP binding protein., Buglino J, Shen V, Hakimian P, Lima CD, Structure. 2002 Nov;10(11):1581-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12429099 12429099]
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Buglino, J.]]
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[[Category: Buglino J]]
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[[Category: Burley, S K.]]
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[[Category: Burley SK]]
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[[Category: Hakimian, P.]]
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[[Category: Hakimian P]]
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[[Category: Lima, C D.]]
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[[Category: Lima CD]]
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[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
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[[Category: Shen V]]
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[[Category: Shen, V.]]
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[[Category: G4P]]
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[[Category: MG]]
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[[Category: gtpase]]
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[[Category: large g-protein]]
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[[Category: new york structural genomix research consortium]]
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[[Category: nysgxrc]]
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[[Category: obg]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: sporulation]]
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[[Category: stress response]]
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[[Category: stringent factor]]
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[[Category: structural genomics]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:47 2008''
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Current revision

Structure of the Obg GTP-binding protein

PDB ID 1lnz

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