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1lo3

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[[Image:1lo3.gif|left|200px]]
 
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{{Structure
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==Retro-Diels-Alderase Catalytic Antibody: Product Analogue==
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|PDB= 1lo3 |SIZE=350|CAPTION= <scene name='initialview01'>1lo3</scene>, resolution 2.30&Aring;
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<StructureSection load='1lo3' size='340' side='right'caption='[[1lo3]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=AN1:3-(10-METHYL-ANTHRACEN-9-YL)-PROPIONIC+ACID'>AN1</scene>
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<table><tr><td colspan='2'>[[1lo3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LO3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LO3 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AN1:3-(10-METHYL-ANTHRACEN-9-YL)-PROPIONIC+ACID'>AN1</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lo3 OCA], [https://pdbe.org/1lo3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lo3 RCSB], [https://www.ebi.ac.uk/pdbsum/1lo3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lo3 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1l00|1L00]], [[1l02|1L02]], [[1l06|1L06]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lo3 OCA], [http://www.ebi.ac.uk/pdbsum/1lo3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lo3 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q65ZC0_MOUSE Q65ZC0_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lo/1lo3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lo3 ConSurf].
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<div style="clear:both"></div>
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'''Retro-Diels-Alderase Catalytic Antibody: Product Analogue'''
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The nitroxyl synthase catalytic antibodies 10F11, 9D9, and 27C5 catalyze the release of nitroxyl from a bicyclic pro-drug by accelerating a retro-Diels-Alder reaction. The Fabs (antigen-binding fragments) of these three catalytic antibodies were cloned and sequenced. Fab 9D9 was crystallized in the apo-form and in complex with one transition state analogue of the reaction. Crystal structures of Fab 10F11 in complex with ligands mimicking substrate, transition state, and product have been determined at resolutions ranging from 1.8 to 2.3 A. Antibodies 9D9 and 10F11 show increased shape complementarity (as quantified by the program sc) to the hapten and to a modeled transition state as compared with substrate and product. The shape complementarity is mediated to a large extent by an aromatic residue (tyrosine or tryptophan) at the bottom of the hydrophobic active pocket, which undergoes pi-stacking interactions with the aromatic rings of the ligands. Another factor contributing to the different reactivity of the regioisomers probably arises because of hydrogen-bonding interactions between the nitroxyl bridge and the backbone amide of PheH101 and possibly a conserved water molecule.
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[[Category: Large Structures]]
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==About this Structure==
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1LO3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LO3 OCA].
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==Reference==
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A structural basis for the activity of retro-Diels-Alder catalytic antibodies: evidence for a catalytic aromatic residue., Hugot M, Bensel N, Vogel M, Reymond MT, Stadler B, Reymond JL, Baumann U, Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9674-8. Epub 2002 Jul 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12093912 12093912]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Baumann U]]
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[[Category: Baumann, U.]]
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[[Category: Hugot M]]
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[[Category: Hugot, M.]]
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[[Category: Reymond JL]]
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[[Category: Reymond, J L.]]
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[[Category: catalytic antibody]]
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[[Category: fab]]
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[[Category: product complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:05:09 2008''
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Current revision

Retro-Diels-Alderase Catalytic Antibody: Product Analogue

PDB ID 1lo3

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