This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1lsi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1lsi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lsi" /> '''LSIII (NMR, 23 STRUCTURES)'''<br /> ==Overv...)
Current revision (08:27, 10 April 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1lsi.gif|left|200px]]<br /><applet load="1lsi" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1lsi" />
 
-
'''LSIII (NMR, 23 STRUCTURES)'''<br />
 
-
==Overview==
+
==LSIII (NMR, 23 STRUCTURES)==
-
We report the sequence-specific proton assignments and solution structure, of the long neurotoxin LSIII from the venom of Laticauda semifasciata, determined by two- and three-dimensional 1H NMR. Input for structure, calculations consisted of 497 NOE-derived distance restraints and 45, dihedral angle restraints obtained from J couplings. A, two-particle-per-residue representation of protein structure was used to, generate 200 initial structures which were then subjected to all-atom, refinement by simulated annealing. Twenty-three final structures, consistent with the experimental restraints were obtained; the average, atomic RMS difference between the individual structures and the mean, structure was 0.82 A for the backbone heavy atoms and 1.3 A for all heavy, atoms (residues 1-26, 37-60). The main elements of regular secondary, structure are a three-stranded antiparallel beta-sheet and three, finger-like loops protruding from a globular core, consistent with, previously reported structures of long neurotoxins. The end of the, prominent loop II, which is involved in binding to acetylcholine receptor, is disordered relative to the rest of the molecule. A novel finding of, this study is that the loop has a well defined local structure; this and, other observations suggest this region moves as a rigid body. We propose, that this motion is a heretofore unrecognized general feature of long, neurotoxins, with specific consequences for binding to the acetylcholine, receptor.
+
<StructureSection load='1lsi' size='340' side='right'caption='[[1lsi]]' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1lsi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LSI FirstGlance]. <br>
-
1LSI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LSI OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lsi OCA], [https://pdbe.org/1lsi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lsi RCSB], [https://www.ebi.ac.uk/pdbsum/1lsi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lsi ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Solution structure of LSIII, a long neurotoxin from the venom of Laticauda semifasciata., Connolly PJ, Stern AS, Hoch JC, Biochemistry. 1996 Jan 16;35(2):418-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8555211 8555211]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/3L21_LATSE 3L21_LATSE] Binds with high affinity to muscular (tested on Torpedo marmorata, Kd=1.6 nM) and neuronal (chimeric alpha-7/CHRNA7, Kd=3 nM) nicotinic acetylcholine receptor (nAChR) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular and neuronal transmission (PubMed:9305882). Also shows a very weak inhibition on GABA(A) receptors (PubMed:26221036). The toxin (10 uM) inhibits 83% of current in channels composed of alpha-1-beta-3-gamma-2 (GABRA1-GABRB3-GABRG2) subunits, 39% of current in channels composed of alpha-2-beta-2-gamma-2 (GABRA2-GABRB2-GABRG2) subunits, and 33% of current in channels composed of alpha-5-beta-2-gamma-2 (GABRA5-GABRB2-GABRG2) subunits (PubMed:26221036).<ref>PMID:26221036</ref> <ref>PMID:9305882</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ls/1lsi_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lsi ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Laticauda semifasciata]]
[[Category: Laticauda semifasciata]]
-
[[Category: Single protein]]
+
[[Category: Connolly PJ]]
-
[[Category: Connolly, P.J.]]
+
[[Category: Hoch JC]]
-
[[Category: Hoch, J.C.]]
+
[[Category: Stern AS]]
-
[[Category: Stern, A.S.]]
+
-
[[Category: multigene family]]
+
-
[[Category: venom]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:50:30 2007''
+

Current revision

LSIII (NMR, 23 STRUCTURES)

PDB ID 1lsi

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1lsi"
Personal tools