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1luz

From Proteopedia

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[[Image:1luz.jpg|left|200px]]<br /><applet load="1luz" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1luz, resolution 1.8&Aring;" />
 
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'''Crystal Structure of the K3L Protein From Vaccinia Virus (Wisconsin Strain)'''<br />
 
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==Overview==
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==Crystal Structure of the K3L Protein From Vaccinia Virus (Wisconsin Strain)==
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The vaccinia virus protein K3L subverts the mammalian antiviral defense mechanism by inhibiting the RNA-dependent protein kinase PKR. K3L is a structural mimic of PKR's natural substrate, the translation initiation factor eIF2alpha. To further our understanding of K3L inhibitory function and PKR substrate recognition, we have solved the 1.8 A X-ray crystal structure of K3L. The structure consists of a five-strand beta barrel with an intervening helix insert region similar in topology to the functionally divergent S1 domain. Mutational analysis identifies two proximal regions of the K3L structure as possessing specialized PKR binding and inhibitory function. Further analysis reveals that PKR dimerization composes a key switch that regulates both its catalytic activation and its molecular recognition of K3L and eIF2alpha.
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<StructureSection load='1luz' size='340' side='right'caption='[[1luz]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1luz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LUZ FirstGlance]. <br>
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1LUZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUZ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1luz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1luz OCA], [https://pdbe.org/1luz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1luz RCSB], [https://www.ebi.ac.uk/pdbsum/1luz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1luz ProSAT]</span></td></tr>
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X-ray crystal structure and functional analysis of vaccinia virus K3L reveals molecular determinants for PKR subversion and substrate recognition., Dar AC, Sicheri F, Mol Cell. 2002 Aug;10(2):295-305. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12191475 12191475]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Vaccinia virus]]
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[https://www.uniprot.org/uniprot/K3_VACCW K3_VACCW] Viral mimic of eIF-2-alpha that acts as a pseudosubstrate for EIF2AK2/PKR kinase. Inhibits therefore eIF-2-alpha phosphorylation by host EIF2AK2/PKR kinase and prevents protein synthesis shutoff.<ref>PMID:8099586</ref>
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[[Category: Dar, A C.]]
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== Evolutionary Conservation ==
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[[Category: Sicheri, F.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: 5 stranded anti-parallel beta barrel]]
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Check<jmol>
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<jmolCheckbox>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:42 2008''
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lu/1luz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1luz ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Vaccinia virus WR]]
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[[Category: Dar AC]]
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[[Category: Sicheri F]]

Current revision

Crystal Structure of the K3L Protein From Vaccinia Virus (Wisconsin Strain)

PDB ID 1luz

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