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1lvb

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(New page: 200px<br /><applet load="1lvb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lvb, resolution 2.20&Aring;" /> '''CATALYTICALLY INACTI...)
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[[Image:1lvb.gif|left|200px]]<br /><applet load="1lvb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lvb, resolution 2.20&Aring;" />
 
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'''CATALYTICALLY INACTIVE TOBACCO ETCH VIRUS PROTEASE COMPLEXED WITH SUBSTRATE'''<br />
 
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==Overview==
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==CATALYTICALLY INACTIVE TOBACCO ETCH VIRUS PROTEASE COMPLEXED WITH SUBSTRATE==
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Because of its stringent sequence specificity, the 3C-type protease from, tobacco etch virus (TEV) is frequently used to remove affinity tags from, recombinant proteins. It is unclear, however, exactly how TEV protease, recognizes its substrates with such high selectivity. The crystal, structures of two TEV protease mutants, inactive C151A and, autolysis-resistant S219D, have now been solved at 2.2- and 1.8-A, resolution as complexes with a substrate and product peptide, respectively. The enzyme does not appear to have been perturbed by the, mutations in either structure, and the modes of binding of the product and, substrate are virtually identical. Analysis of the protein-ligand, interactions helps to delineate the structural determinants of substrate, specificity and provides guidance for reengineering the enzyme to further, improve its utility for biotechnological applications.
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<StructureSection load='1lvb' size='340' side='right'caption='[[1lvb]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lvb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Tobacco_etch_virus Tobacco etch virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LVB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvb OCA], [https://pdbe.org/1lvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lvb RCSB], [https://www.ebi.ac.uk/pdbsum/1lvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lvb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/POLG_TEV POLG_TEV] Capsid protein: involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.<ref>PMID:9880030</ref> <ref>PMID:11414807</ref> Nuclear inclusion protein B: an RNA-dependent RNA polymerase that plays an essential role in the virus replication.<ref>PMID:9880030</ref> <ref>PMID:11414807</ref> Helper component proteinase: required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity.<ref>PMID:9880030</ref> <ref>PMID:11414807</ref> Cytoplasmic inclusion protein: has helicase activity. It may be involved in replication.<ref>PMID:9880030</ref> <ref>PMID:11414807</ref> Both 6K peptides are indispensable for virus replication (By similarity).<ref>PMID:9880030</ref> <ref>PMID:11414807</ref> Nuclear inclusion protein A: has RNA-binding and proteolytic activities.<ref>PMID:9880030</ref> <ref>PMID:11414807</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lv/1lvb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lvb ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LVB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Tobacco_etch_virus Tobacco etch virus] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LVB OCA].
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*[[Tobacco Etch Virus (TEV) Protease|Tobacco Etch Virus (TEV) Protease]]
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== References ==
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==Reference==
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<references/>
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Structural basis for the substrate specificity of tobacco etch virus protease., Phan J, Zdanov A, Evdokimov AG, Tropea JE, Peters HK 3rd, Kapust RB, Li M, Wlodawer A, Waugh DS, J Biol Chem. 2002 Dec 27;277(52):50564-72. Epub 2002 Oct 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12377789 12377789]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Tobacco etch virus]]
[[Category: Tobacco etch virus]]
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[[Category: Evdokimov, A.G.]]
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[[Category: Evdokimov AG]]
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[[Category: III, H.K.Peters.]]
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[[Category: Kapust RB]]
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[[Category: Kapust, R.B.]]
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[[Category: Li M]]
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[[Category: Li, M.]]
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[[Category: Peters III HK]]
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[[Category: Phan, J.]]
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[[Category: Phan J]]
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[[Category: Tropea, J.E.]]
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[[Category: Tropea JE]]
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[[Category: Waugh, D.S.]]
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[[Category: Waugh DS]]
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[[Category: Wlodawer, A.]]
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[[Category: Wlodawer A]]
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[[Category: Zdanov, A.]]
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[[Category: Zdanov A]]
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[[Category: GOL]]
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[[Category: beta barrel]]
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[[Category: chymotrypsin-like cystein protease]]
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[[Category: protein-peptide complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:55:02 2007''
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Current revision

CATALYTICALLY INACTIVE TOBACCO ETCH VIRUS PROTEASE COMPLEXED WITH SUBSTRATE

PDB ID 1lvb

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