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1lvh

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The Structure of Phosphorylated beta-phosphoglucomutase from Lactoccocus lactis to 2.3 angstrom resolution

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-[[Image:1lvh.gif|left|200px]]<br /><applet load="1lvh" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1lvh, resolution 2.30&Aring;" />
-'''The Structure of Phosphorylated beta-phosphoglucomutase from Lactoccocus lactis to 2.3 angstrom resolution'''<br />
-==Overview==
+==The Structure of Phosphorylated beta-phosphoglucomutase from Lactoccocus lactis to 2.3 angstrom resolution==
-Phosphoglucomutases catalyze the interconversion of D-glucose 1-phosphate, and D-glucose 6-phosphate, a reaction central to energy metabolism in all, cells and to the synthesis of cell wall polysaccharides in bacterial, cells. Two classes of phosphoglucomutases (alpha-PGM and beta-PGM) are, distinguished on the basis of their specificity for alpha- and, beta-glucose-1-phosphate. beta-PGM is a member of the haloacid, dehalogenase (HAD) superfamily, which includes the sarcoplasmic, Ca(2+)-ATPase, phosphomannomutase, and phosphoserine phosphatase. beta-PGM, is unusual among family members in that the common phosphoenzyme, intermediate exists as a stable ground-state complex in this enzyme., Herein we report, for the first time, the three-dimensional structure of a, beta-PGM and the first view of the true phosphoenzyme intermediate in the, HAD superfamily. The crystal structure of the Mg(II) complex of, phosphorylated beta-phosphoglucomutase (beta-PGM) from Lactococcus lactis, has been determined to 2.3 A resolution by multiwavelength anomalous, diffraction (MAD) phasing on selenomethionine, and refined to an R(cryst), = 0.24 and R(free) = 0.28. The active site of beta-PGM is located between, the core and the cap domain and is freely solvent accessible. The residues, within a 6 A radius of the phosphorylated Asp8 include Asp10, Thr16, Ser114, Lys145, Glu169, and Asp170. The cofactor Mg(2+) is liganded with, octahedral coordination geometry by the carboxylate side chains of Asp8, Glu169, Asp170, and the backbone carbonyl oxygen of Asp10 along with one, oxygen from the Asp8-phosphoryl group and one water ligand. The phosphate, group of the phosphoaspartyl residue, Asp8, interacts with the side chains, of Ser114 and Lys145. The absence of a base residue near the aspartyl, phosphate group accounts for the persistence of the phosphorylated enzyme, under physiological conditions. Substrate docking shows that glucose-6-P, can bind to the active site of phosphorylated beta-PGM in such a way as to, position the C(1)OH near the phosphoryl group of the phosphorylated Asp8, and the C(6) phosphoryl group near the carboxylate group of Asp10. This, result suggests a novel two-base mechanism for phosphoryl group transfer, in a phosphorylated sugar.
+<StructureSection load='1lvh' size='340' side='right'caption='[[1lvh]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1lvh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LVH FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PHD:ASPARTYL+PHOSPHATE'>PHD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lvh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvh OCA], [https://pdbe.org/1lvh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lvh RCSB], [https://www.ebi.ac.uk/pdbsum/1lvh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lvh ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PGMB_LACLA PGMB_LACLA] Catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C(1) anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose.<ref>PMID:9084169</ref> <ref>PMID:15005616</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lv/1lvh_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lvh ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-LVH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LVH OCA].
+*[[Beta-phosphoglucomutase|Beta-phosphoglucomutase]]
+*[[Beta-phosphoglucomutase 3D structures|Beta-phosphoglucomutase 3D structures]]
-==Reference==
+== References ==
-Caught in the act: the structure of phosphorylated beta-phosphoglucomutase from Lactococcus lactis., Lahiri SD, Zhang G, Dunaway-Mariano D, Allen KN, Biochemistry. 2002 Jul 2;41(26):8351-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12081483 12081483]
+<references/>
-[[Category: Beta-phosphoglucomutase]]
+__TOC__
+</StructureSection>
 [[Category: Lactococcus lactis]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Allen, K.N.]]
+[[Category: Allen KN]]
-[[Category: Dunaway-Mariano, D.]]
+[[Category: Dunaway-Mariano D]]
-[[Category: Lahiri, S.D.]]
+[[Category: Lahiri SD]]
-[[Category: Zhang, G.]]
+[[Category: Zhang G]]
-[[Category: MG]]
-[[Category: aspartylphosphate]]
-[[Category: had superfamily]]
-[[Category: phosphoaspartate]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:55:17 2007''

1lvh

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The Structure of Phosphorylated beta-phosphoglucomutase from Lactoccocus lactis to 2.3 angstrom resolution

Structural highlights

Function

Evolutionary Conservation

See Also

References

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