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1m1d

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[[Image:1m1d.jpg|left|200px]]
 
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{{Structure
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==TETRAHYMENA GCN5 WITH BOUND BISUBSTRATE ANALOG INHIBITOR==
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|PDB= 1m1d |SIZE=350|CAPTION= <scene name='initialview01'>1m1d</scene>, resolution 2.2&Aring;
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<StructureSection load='1m1d' size='340' side='right'caption='[[1m1d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=LYX:N&#39;&#39;-(2-COENZYME+A)-PROPANOYL-LYSINE'>LYX</scene>
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<table><tr><td colspan='2'>[[1m1d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M1D FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LYX:N-(2-COENZYME+A)-PROPANOYL-LYSINE'>LYX</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m1d OCA], [https://pdbe.org/1m1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m1d RCSB], [https://www.ebi.ac.uk/pdbsum/1m1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m1d ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1qsn|1QSN]], [[1qsr|1QSR]], [[1qst|1QST]], [[1ygh|1YGH]], [[1cmo|1CMO]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m1d OCA], [http://www.ebi.ac.uk/pdbsum/1m1d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1m1d RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/H3_YEAST H3_YEAST]
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== Evolutionary Conservation ==
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'''TETRAHYMENA GCN5 WITH BOUND BISUBSTRATE ANALOG INHIBITOR'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m1/1m1d_consurf.spt"</scriptWhenChecked>
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Histone acetyltransferases (HATs) use acetyl CoA to acetylate target lysine residues within histones and other transcription factors, such as the p53 tumor suppressor, to promote gene activation. HAT enzymes fall into subfamilies with divergence in sequence and substrate preference. Several HAT proteins have been implicated in human cancer. We have previously reported on the preparation of peptide-CoA conjugate inhibitors with distinct specificities for the p300/CBP [cAMP response element binding protein (CREB)-binding protein] or GCN5 HAT subfamilies. Here we report on the crystal structure of the GCN5 HAT bound to a peptide-CoA conjugate containing CoA covalently attached through an isopropionyl linker to Lys-14 of a 20-aa N-terminal fragment of histone H3. Surprisingly, the structure reveals that the H3 portion of the inhibitor is bound outside of the binding site for the histone substrate and that only five of the 20 aa residues of the inhibitor are ordered. Rearrangements within the C-terminal region of the GCN5 protein appear to mediate this peptide displacement. Mutational and enzymatic data support the hypothesis that the observed structure corresponds to a late catalytic intermediate. The structure also provides a structural scaffold for the design of HAT-specific inhibitors that may have therapeutic applications for the treatment of HAT-mediated cancers.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1M1D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M1D OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m1d ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structure of the GCN5 histone acetyltransferase bound to a bisubstrate inhibitor., Poux AN, Cebrat M, Kim CM, Cole PA, Marmorstein R, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14065-70. Epub 2002 Oct 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12391296 12391296]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Tetrahymena thermophila]]
[[Category: Tetrahymena thermophila]]
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[[Category: Cebrat, M.]]
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[[Category: Cebrat M]]
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[[Category: Cole, P A.]]
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[[Category: Cole PA]]
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[[Category: Kim, C M.]]
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[[Category: Kim CM]]
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[[Category: Marmorstein, R.]]
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[[Category: Marmorstein R]]
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[[Category: Poux, A N.]]
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[[Category: Poux AN]]
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[[Category: gcn5-related n-acetyltransferase]]
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[[Category: histone acetyltransferase]]
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[[Category: inhibitor complex]]
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[[Category: transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:10:03 2008''
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Current revision

TETRAHYMENA GCN5 WITH BOUND BISUBSTRATE ANALOG INHIBITOR

PDB ID 1m1d

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