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1m32

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(New page: 200px<br /><applet load="1m32" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m32, resolution 2.2&Aring;" /> '''Crystal Structure of ...)
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[[Image:1m32.gif|left|200px]]<br /><applet load="1m32" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m32, resolution 2.2&Aring;" />
 
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'''Crystal Structure of 2-aminoethylphosphonate Transaminase'''<br />
 
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==Overview==
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==Crystal Structure of 2-aminoethylphosphonate Transaminase==
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Phosphonates allow certain organisms to thrive in otherwise hostile, environments, and 2-aminoethylphosphonate (AEP) is a precursor of many, cellular phosphonates. AEP transaminase (AEPT) is an enzyme essential to, phosphonate synthesis and degradation pathways. The crystal structure of, AEP transaminase was determined by multiwavelength anomalous diffraction, of 66 selenium atoms. The refined structure at 2.2 A resolution revealed, an overall fold and active site location similar to those of the dimeric, two-domain structure of type I aminotransferases. The active site contains, a cofactor, pyridoxal 5'-phosphate (PLP), and the product, phosphonoacetaldehyde. Comparison with other type I aminotransferase, structures shows that the PLP-protein interactions are conserved. Modeling, of bound substrates and products reveals the structural basis for AEP, recognition and the stereospecificity of proton elimination at the, alpha-carbon and indicates conformational changes along the reaction, pathway.
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<StructureSection load='1m32' size='340' side='right'caption='[[1m32]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1m32]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M32 FirstGlance]. <br>
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1M32 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with PO4, PLP and POA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/2-aminoethylphosphonate--pyruvate_transaminase 2-aminoethylphosphonate--pyruvate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.37 2.6.1.37] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M32 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=POA:PHOSPHONOACETALDEHYDE'>POA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m32 OCA], [https://pdbe.org/1m32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m32 RCSB], [https://www.ebi.ac.uk/pdbsum/1m32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m32 ProSAT]</span></td></tr>
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Degradation pathway of the phosphonate ciliatine: crystal structure of 2-aminoethylphosphonate transaminase., Chen CC, Zhang H, Kim AD, Howard A, Sheldrick GM, Mariano-Dunaway D, Herzberg O, Biochemistry. 2002 Nov 5;41(44):13162-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12403617 12403617]
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</table>
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[[Category: 2-aminoethylphosphonate--pyruvate transaminase]]
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== Function ==
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[[Category: Salmonella typhimurium]]
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[https://www.uniprot.org/uniprot/PHNW_SALTY PHNW_SALTY] Involved in phosphonate degradation.
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Chen, C.C.H.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Herzberg, O.]]
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Check<jmol>
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[[Category: Howard, A.]]
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<jmolCheckbox>
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[[Category: Kim, A.D.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m3/1m32_consurf.spt"</scriptWhenChecked>
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[[Category: Mariano-Dunnaway, D.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Sheldrick, G.M.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Zhang, H.]]
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</jmolCheckbox>
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[[Category: PLP]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m32 ConSurf].
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[[Category: PO4]]
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<div style="clear:both"></div>
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[[Category: POA]]
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__TOC__
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[[Category: plp-dependent aminotransferase fold]]
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</StructureSection>
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[[Category: Large Structures]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:06:24 2007''
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Chen CCH]]
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[[Category: Herzberg O]]
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[[Category: Howard A]]
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[[Category: Kim AD]]
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[[Category: Mariano-Dunnaway D]]
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[[Category: Sheldrick GM]]
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[[Category: Zhang H]]

Current revision

Crystal Structure of 2-aminoethylphosphonate Transaminase

PDB ID 1m32

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