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1m9u

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[[Image:1m9u.jpg|left|200px]]
 
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==Crystal Structure of Earthworm Fibrinolytic Enzyme Component A from Eisenia fetida==
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The line below this paragraph, containing "STRUCTURE_1m9u", creates the "Structure Box" on the page.
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<StructureSection load='1m9u' size='340' side='right'caption='[[1m9u]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1m9u]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Eisenia_fetida Eisenia fetida]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ij7 1ij7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M9U FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9u OCA], [https://pdbe.org/1m9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m9u RCSB], [https://www.ebi.ac.uk/pdbsum/1m9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9u ProSAT]</span></td></tr>
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{{STRUCTURE_1m9u| PDB=1m9u | SCENE= }}
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</table>
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== Function ==
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'''Crystal Structure of Earthworm Fibrinolytic Enzyme Component A from Eisenia fetida'''
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[https://www.uniprot.org/uniprot/Q8MX72_EISFE Q8MX72_EISFE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Earthworm fibrinolytic enzyme component A (EFEa) from Eisenia fetida is a strong fibrinolytic enzyme that not only directly degrades fibrin, but also activates plasminogen. Proteolytic assays further revealed that it cleaved behind various P1 residue types. The crystal structure of EFEa was determined using the MIR method and refined to 2.3A resolution. The enzyme, showing the overall polypeptide fold of chymotrypsin-like serine proteases, possesses essential S1 specificity determinants characteristic of elastase. However, the beta strand at the west rim of the S1 specificity pocket is significantly elongated by a unique four-residue insertion (Ser-Ser-Gly-Leu) after Val217, which not only provides additional substrate hydrogen binding sites for distal P residues, but also causes extension of the S1 pocket at the south rim. The S2 subsite of the enzyme was partially occluded by the bulky side-chain of residue Tyr99. Structure-based inhibitor modeling demonstrated that EFEa's S1 specificity pocket was preferable for elastase-specific small hydrophobic P1 residues, while its accommodation of long and/or bulky P1 residues was also feasible if enhanced binding of the substrate and induced fit of the S1 pocket were achieved. EFEa is thereby endowed with relatively broad substrate specificity, including the dual fibrinolysis. The presence of Tyr99 at the S2 subsite indicates a preference for P2-Gly, while an induced fit of Tyr99 was also suggested for accommodation of bigger P2 residues. This structure is the first reported for an earthworm fibrinolytic enzyme component and serine protease originating from annelid worms.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m9/1m9u_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1M9U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Eisenia_fetida Eisenia fetida]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ij7 1ij7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9U OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m9u ConSurf].
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Crystal structure of earthworm fibrinolytic enzyme component a: revealing the structural determinants of its dual fibrinolytic activity., Tang Y, Liang D, Jiang T, Zhang J, Gui L, Chang W, J Mol Biol. 2002 Aug 2;321(1):57-68. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12139933 12139933]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Eisenia fetida]]
[[Category: Eisenia fetida]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chang, W.]]
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[[Category: Chang W]]
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[[Category: Liang, D.]]
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[[Category: Liang D]]
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[[Category: Tang, Y.]]
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[[Category: Tang Y]]
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[[Category: Fibrinolytic enzyme]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:48:24 2008''
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Current revision

Crystal Structure of Earthworm Fibrinolytic Enzyme Component A from Eisenia fetida

PDB ID 1m9u

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