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1mba

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(New page: 200px<br /><applet load="1mba" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mba, resolution 1.6&Aring;" /> '''APLYSIA LIMACINA MYOG...)
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[[Image:1mba.jpg|left|200px]]<br /><applet load="1mba" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mba, resolution 1.6&Aring;" />
 
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'''APLYSIA LIMACINA MYOGLOBIN. CRYSTALLOGRAPHIC ANALYSIS AT 1.6 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==APLYSIA LIMACINA MYOGLOBIN. CRYSTALLOGRAPHIC ANALYSIS AT 1.6 ANGSTROMS RESOLUTION==
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The crystal structure of the ferric form of myoglobin from the mollusc, Aplysia limacina has been refined at 1.6 A resolution, by restrained, crystallographic refinement methods. The crystallographic R-factor is, 0.19. The tertiary structure of the molecule conforms to the common globin, fold, consisting of eight alpha-helices. The N-terminal helix A and helix, G deviate significantly from linearity. The distal residue is recognized, as Val63 (E7), which, however, does not contact the heme directly., Moreover the sixth (distal) co-ordination position of heme iron is not, occupied by a water molecule at neutrality, i.e. below the acid-alkaline, transition point of A. limacina myoglobin. The heme group sits in its, crevice in the conventional orientation and no signs of heme isomerism are, evident. The iron atom is 0.26 A out of the porphyrin plane, with a mean, Fe-N (porphyrin) distance of 2.01 A. The co-ordination bond to the, proximal histidine has a length of 2.05 A, and forms an angle of 4 degrees, with the heme normal. A plane containing the imidazole ring of the, proximal His intersects the heme at an angle of 29 degrees with the, (porphyrin) 4N-2N direction. Inspection of the structure of pH 9.0, indicates that a hydroxyl ion is bound to the Fe sixth co-ordination, position.
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<StructureSection load='1mba' size='340' side='right'caption='[[1mba]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mba]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_limacina Aplysia limacina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MBA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MBA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mba OCA], [https://pdbe.org/1mba PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mba RCSB], [https://www.ebi.ac.uk/pdbsum/1mba PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mba ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLB_APLLI GLB_APLLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mb/1mba_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mba ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MBA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_limacina Aplysia limacina] with ACE and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MBA OCA].
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*[[Myoglobin 3D structures|Myoglobin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Aplysia limacina myoglobin. Crystallographic analysis at 1.6 A resolution., Bolognesi M, Onesti S, Gatti G, Coda A, Ascenzi P, Brunori M, J Mol Biol. 1989 Feb 5;205(3):529-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2926816 2926816]
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[[Category: Aplysia limacina]]
[[Category: Aplysia limacina]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ascenzi, P.]]
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[[Category: Ascenzi P]]
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[[Category: Bolognesi, M.]]
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[[Category: Bolognesi M]]
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[[Category: Brunori, M.]]
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[[Category: Brunori M]]
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[[Category: Coda, A.]]
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[[Category: Coda A]]
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[[Category: Gatti, G.]]
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[[Category: Gatti G]]
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[[Category: Onesti, S.]]
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[[Category: Onesti S]]
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[[Category: ACE]]
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[[Category: HEM]]
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[[Category: oxygen storage]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:17:43 2007''
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Current revision

APLYSIA LIMACINA MYOGLOBIN. CRYSTALLOGRAPHIC ANALYSIS AT 1.6 ANGSTROMS RESOLUTION

PDB ID 1mba

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