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1md8

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[[Image:1md8.gif|left|200px]]<br /><applet load="1md8" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1md8, resolution 2.80&Aring;" />
 
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'''Monomeric structure of the active catalytic domain of complement protease C1r'''<br />
 
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==Overview==
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==Monomeric structure of the active catalytic domain of complement protease C1r==
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C1r is the serine protease (SP) that mediates autoactivation of C1, the complex that triggers the classical complement pathway. We have determined the crystal structure of two fragments from the human C1r catalytic domain, each encompassing the second complement control protein (CCP2) module and the SP domain. The wild-type species has an active structure, whereas the S637A mutant is a zymogen. The structures reveal a restricted hinge flexibility of the CCP2-SP interface, and both are characterized by the unique alpha-helical conformation of loop E. The zymogen activation domain exhibits high mobility, and the active structure shows a restricted access to most substrate binding subsites. Further implications relevant to the C1r self-activation process are derived from protein-protein interactions in the crystals.
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<StructureSection load='1md8' size='340' side='right'caption='[[1md8]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==Disease==
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<table><tr><td colspan='2'>[[1md8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MD8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MD8 FirstGlance]. <br>
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Known disease associated with this structure: C1r/C1s deficiency, combined OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=216950 216950]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1md8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1md8 OCA], [https://pdbe.org/1md8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1md8 RCSB], [https://www.ebi.ac.uk/pdbsum/1md8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1md8 ProSAT]</span></td></tr>
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==About this Structure==
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</table>
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1MD8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Complement_subcomponent_C1r Complement subcomponent C1r], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.41 3.4.21.41] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MD8 OCA].
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== Function ==
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[https://www.uniprot.org/uniprot/C1R_HUMAN C1R_HUMAN] C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
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==Reference==
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== Evolutionary Conservation ==
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Monomeric structures of the zymogen and active catalytic domain of complement protease c1r: further insights into the c1 activation mechanism., Budayova-Spano M, Grabarse W, Thielens NM, Hillen H, Lacroix M, Schmidt M, Fontecilla-Camps JC, Arlaud GJ, Gaboriaud C, Structure. 2002 Nov;10(11):1509-19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12429092 12429092]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Complement subcomponent C1r]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/md/1md8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1md8 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arlaud, G J.]]
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[[Category: Arlaud GJ]]
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[[Category: Budayova-Spano, M.]]
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[[Category: Budayova-Spano M]]
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[[Category: Fontecilla-Camps, J.]]
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[[Category: Fontecilla-Camps J]]
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[[Category: Gaboriaud, C.]]
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[[Category: Gaboriaud C]]
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[[Category: Grabarse, W.]]
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[[Category: Grabarse W]]
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[[Category: Hillen, H.]]
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[[Category: Hillen H]]
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[[Category: Lacroix, M.]]
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[[Category: Lacroix M]]
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[[Category: Schmidt, M.]]
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[[Category: Schmidt M]]
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[[Category: Thielens, N M.]]
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[[Category: Thielens NM]]
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[[Category: activation]]
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[[Category: complement]]
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[[Category: innate immunity]]
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[[Category: serine protease]]
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[[Category: substrate specificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:54:06 2008''
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Current revision

Monomeric structure of the active catalytic domain of complement protease C1r

PDB ID 1md8

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