This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1me3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:34, 10 April 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1me3.jpg|left|200px]]
 
-
{{Structure
+
==High Resolution Crystal Structure Analysis Of Cruzain non-covalently Bound To A Hydroxymethyl Ketone Inhibitor (II)==
-
|PDB= 1me3 |SIZE=350|CAPTION= <scene name='initialview01'>1me3</scene>, resolution 1.2&Aring;
+
<StructureSection load='1me3' size='340' side='right'caption='[[1me3]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=P10:[1-(3-HYDROXY-2-OXO-1-PHENETHYL-PROPYLCARBAMOYL)2-PHENYL-ETHYL]-CARBAMIC ACID PYRIDIN-4-YLMETHYL ESTER'>P10</scene>
+
<table><tr><td colspan='2'>[[1me3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ME3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ME3 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P10:[1-(3-HYDROXY-2-OXO-1-PHENETHYL-PROPYLCARBAMOYL)2-PHENYL-ETHYL]-CARBAMIC+ACID+PYRIDIN-4-YLMETHYL+ESTER'>P10</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1me3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1me3 OCA], [https://pdbe.org/1me3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1me3 RCSB], [https://www.ebi.ac.uk/pdbsum/1me3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1me3 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CYSP_TRYCR CYSP_TRYCR] Hydrolyzes chromogenic peptides at the carboxyl Arg or Lys; requires at least one more amino acid, preferably Arg, Phe, Val or Leu, between the terminal Arg or Lys and the amino-blocking group. The cysteine protease may play an important role in the development and differentiation of the parasites at several stages of their life cycle.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/me/1me3_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1me3 ConSurf].
 +
<div style="clear:both"></div>
-
'''High Resolution Crystal Structure Analysis Of Cruzain non-covalently Bound To A Hydroxymethyl Ketone Inhibitor (II)'''
+
==See Also==
-
 
+
*[[Cruzain|Cruzain]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The crystal structures of two hydroxymethyl ketone inhibitors complexed to the cysteine protease cruzain have been determined at 1.1 and 1.2 A resolution, respectively. These high resolution crystal structures provide the first structures of non-covalent inhibitors bound to cruzain. A series of compounds were prepared and tested based upon the structures providing further insight into the key binding interactions.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
1ME3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ME3 OCA].
+
-
 
+
-
==Reference==
+
-
Crystal structures of reversible ketone-Based inhibitors of the cysteine protease cruzain., Huang L, Brinen LS, Ellman JA, Bioorg Med Chem. 2003 Jan 2;11(1):21-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12467703 12467703]
+
-
[[Category: Single protein]]
+
[[Category: Trypanosoma cruzi]]
[[Category: Trypanosoma cruzi]]
-
[[Category: Brinen, L S.]]
+
[[Category: Brinen LS]]
-
[[Category: Ellman, J A.]]
+
[[Category: Ellman JA]]
-
[[Category: Huang, L.]]
+
[[Category: Huang L]]
-
[[Category: P10]]
+
-
[[Category: cysteine protease]]
+
-
[[Category: non-covalent inhibitor]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:42:23 2008''
+

Current revision

High Resolution Crystal Structure Analysis Of Cruzain non-covalently Bound To A Hydroxymethyl Ketone Inhibitor (II)

PDB ID 1me3

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1me3"
Personal tools