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1meg

From Proteopedia

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[[Image:1meg.gif|left|200px]]<br />
 
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<applet load="1meg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1meg, resolution 2.0&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64==
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The structure of the D158E mutant of caricain (previously known as papaya, protease omega) in complex with E-64 has been determined at 2.0 A, resolution (overall R factor 19.3%). The structure reveals that the, substituted glutamate makes the same pattern of hydrogen bonds as the, aspartate in native caricain. This was not anticipated since in the native, structure there is insufficient room to accommodate the glutamate side, chain. The glutamate is accommodated in the mutant by a local expansion of, the structure demonstrating that small structural changes are responsible, for the change in activity.
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<StructureSection load='1meg' size='340' side='right'caption='[[1meg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1meg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Carica_papaya Carica papaya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MEG FirstGlance]. <br>
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1MEG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya] with E64 and EOH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Caricain Caricain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.30 3.4.22.30] Structure known Active Site: ACT. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MEG OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene>, <scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1meg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1meg OCA], [https://pdbe.org/1meg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1meg RCSB], [https://www.ebi.ac.uk/pdbsum/1meg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1meg ProSAT]</span></td></tr>
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Crystal structure of a caricain D158E mutant in complex with E-64., Katerelos NA, Taylor MA, Scott M, Goodenough PW, Pickersgill RW, FEBS Lett. 1996 Aug 19;392(1):35-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8769310 8769310]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PAPA3_CARPA PAPA3_CARPA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/me/1meg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1meg ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Carica papaya]]
[[Category: Carica papaya]]
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[[Category: Caricain]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Katerelos NA]]
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[[Category: Katerelos, N.A.]]
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[[Category: E64]]
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[[Category: EOH]]
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[[Category: cysteine proteinase]]
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[[Category: hydrolase]]
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[[Category: thiol protease]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:22:33 2007''
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Current revision

CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64

PDB ID 1meg

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